安徽农业大学学报2017，Vol.44Issue(2)：229-233,5.DOI:10.13610/j.cnki.1672-352x.20170419.022
鳙鱼维氏气单胞菌的分离鉴定及其毒力基因检测
Isolation, identification and virulence genes detection of Aeromonas veronii from Aristichthys nobilis
摘要
Abstract
A bacterial strain named AH-YS was isolated from diseased Aristichthys nobilis,which was identified as Aeromonas veronii using a combination of gram stain,oxidase test,biochemical identification,PCR amplification of 16S rRNA and phylogenetic analysis.Drug sensitive test of a total of 11 antimicrobial agents showed that AH-YS was sensitive to 9 drugs.PCR amplification of a total of 9 virulence genes revealed that 5 genes could be detected,which are aerolysin,cytotoxic enterotoxin,flagellin,elastase and lipase.关键词
NaCl胁迫/番茄/种子发芽/耐盐性Key words
Aeromonas veronii/Aristichthys nobilis/isolation and identification/virulence genes分类
农业科技引用本文复制引用
朱若林,马腾,陈露,崔晓羽,张嫣然,孙创,彭开松,张晓华,鲍传和..鳙鱼维氏气单胞菌的分离鉴定及其毒力基因检测[J].安徽农业大学学报,2017,44(2):229-233,5.基金项目
国家自然科学基金(31402331),安徽省现代农业产业技术体系(2016-2020)(皖农科[2016]84号)-水产产业体系,安徽农业大学引进与稳定人才项目(YJ2015-15)和安徽农业大学校级大学生科技创新基金项目(XJ2015176)共同资助. （31402331）
