安徽农业大学学报2017，Vol.44Issue(2)：321-326,6.DOI:10.13610/j.cnki.1672-352x.20170419.008
钼对烤烟不同品种叶绿素荧光、抗氧化及氮代谢酶活性的影响
Effects of molybdenum on chlorophyll fluorescence, antioxidant activity and nitrogen metabolism in flue-cured tobacco of different varieties
摘要
Abstract
We studied the effects of molybdenum (Mo) on the growth,chlorophyll fluorescence,antioxidant activity and the activities of key enzymes involved in nitrogen metabolism in two flue-cured tobacco varieties,Wanhua K326 and Nanjiang 3 with or without Mo in culture solution for 30 days.Results showed that,compared with Mo deficiency,the agronomic traits of Wanhua K326 and Nanjiang 3 with Mo application were affected significantly by increasing the root length,stem girth and fresh weight of 4.48％,26.52％,13.06％ and 7.13％,6.38％,5.52％,respectively.Fm (maximal-fluorescence output) and Fv/Fm (optimal/maximal quantum yield of PS Ⅱ) of the two varieties were also increased by 10.33％,13.70％ and 13.52％,7.01％,respectively with Mo application.The activities of CAT (Cat from micrococcus lysodeikticus),POD (peroxidase),SOD (superoxide dismutase) in Wanhua K326 variety were obviously increased by 145.83％,90.72％,and 5.58％,respectively with Mo application.The SOD activity in Nanjiang 3 increased by 5.76％,and the NR (nitrate reductase) activities in Wanhua K326 and Nanjiang 3 increased by 107.41％ and 16.00％,respectively with Mo application as compared with Mo deficiency.The difference in NR activity between Wanhua K326 and Nanjiang 3 was significant under Mo deficiency condition.The glutamine synthetase (GS) activity increased with Mo application.Of which,Wanhua K326 varieties increased considerably.The GS activity in Nanjiang 3 and Wanhua K326 showed significant difference with Mo application,and the difference among Nanjiang 3 varieties were significant.The nitrite content in Mo application tobacco was lower than that in Mo deficiency,and the difference in nitrite content between Wanhua K326 and Nanjiang 3 was significant under Mo deficiency condition.In summary,Mo application could promote the growth of the two tobacco varieties and increase chlorophyll fluorescence,antioxidant and nitrogen metabolism.The effect of Mo application on Wanhua K326 tobacco variety was more significant than on Nanjiang 3.关键词
钼/烤烟/品种/荧光参数/抗氧化酶/氮代谢Key words
KSAP/humectant of tobacco/moisture retention/humidity resistance/infrared spectrum/scanning electron microscopy分类
农业科技引用本文复制引用
李朝阳,夏志林,芶剑渝,贺方云,黄婷,武丽,李章海..钼对烤烟不同品种叶绿素荧光、抗氧化及氮代谢酶活性的影响[J].安徽农业大学学报,2017,44(2):321-326,6.基金项目
贵州省烟草公司科技项目“黔北(遵义)山地烟关键生产技术研究与应用”(合同号:201612)资助. （遵义）
