安徽农业科学2017，Vol.45Issue(12)：130-131,135,3.
党参无菌苗培养前种子消毒技术研究
Methods for Seed Disinfection before Culturing Aseptic Codonopsis pilosula Seedling
摘要
Abstract
[Objective]To study the disinfection effects of Codonopsis pilosula seedling under different disinfection treatments.[Method]Taking C.pilosula seeds as test materials,through three kinds of disinfectants 10%H2O2, 0.1% HgCl2 and 2% NaClO,pretreatment with 75% ethanol and presoaking with sterile water,the effects of C.pilosula seed sterilization were studied.[Result]2%NaClO had the greatest sterilization effect.The germ-carring rate of seed was decreased with pretreatment of 75% ethanol.The seeds were soaked in aseptic water for 24 hours before sterilization,the seeds germination rate could increase.[Conclusion]The results of this study provide an important basis for the research on the cultivation of sterile seedlings of C.pilosula.关键词
党参/种子消毒/无菌苗Key words
Codonopsis pilosula/Seed disinfection/Aseptic seedling分类
农业科技引用本文复制引用
薛雯心,魏铁锁,袁金月,刘立业,姜雪琪,李秀霞..党参无菌苗培养前种子消毒技术研究[J].安徽农业科学,2017,45(12):130-131,135,3.基金项目
国家级大学生创新创业训练计划项目(201510222009). （201510222009）
