吉林大学学报（医学版）2017，Vol.43Issue(3)：474-478,5.DOI:10.13481/j.1671-587x.20170303
人生长激素释放激素受体剪接变异体1型对人肝癌细胞HepG2增殖的影响
Effect of human growth hormone releasing hormone receptor splice variant type 1 on proliferation of human liver cancer HepG2 cells
摘要
Abstract
Objective:To investigate the effect of human growth hormone releasing hormone receptor splice variant type 1 (GHRHR SV1) on the proliferation of human liver cancer HepG2 cells,and to clarify the proliferation effect of GHRHR SV1 on the human cancer cells.Methods:The GHRHR SV1 plasmids were transfected into the human HepG2 cells to construct the HepG2-SV1 cell line.HepG2 group(HepG2 cells),HepG2-empty group(HepG2-pcDNA3.0 cell line) and HepG2-SV1 group(HepG2-SV1 cells) were set up.PCR and Western blotting methods were used to identify the HepG2-SV1 cell line;CCK-8 method was used to detect prolifernation rate of cells;colony formation assay was used to detect the colony formation rate of cells;cell wound healing assay was used to evaluate the migration rate of cells.Results:The PCR and Western blotting results showed the HepG2-SV1 cell line expressed GHRHR SV1 steadily.The CCK-8 results showed that the proliferation rate of the HepG2-SV1 cells in HepG2-SV1 group was higher than that of the HepG2-pcDNA3.0 cells in HepG2-empty group(P<0.05).The colony formation assay results showed that the colony formation rate of HepG2-SV1 cells in HepG2-SV1 group was 3.5 times higher than that of the HepG2-pcDNA3.0 cells in HepG2-empty group(P<0.05).The cell wound scratch assay results showed that the migration rate of the HepG2-SV1 cells in HepG2-SV1 group was higher than that of the HepG2-pcDNA3.0 cells in HepG2-empty group(P<0.05).Conclusion:GHRHR SV1 could increase the proliferation of HepG2 cells.关键词
人生长激素释放激素受体剪接变异体1型/HepG2细胞/细胞增殖/克隆形成率/迁移率Key words
human growth hormone releasing hormone receptor splice variant type 1/HepG2 cells/cell proliferation/colony formation rate/migration rate分类
医药卫生引用本文复制引用
林玉茵,陈文生,郭晓兰,谭茵,贺小松,戴建威..人生长激素释放激素受体剪接变异体1型对人肝癌细胞HepG2增殖的影响[J].吉林大学学报（医学版）,2017,43(3):474-478,5.基金项目
国家自然科学基金资助课题(81501014) （81501014）
广东省科技厅自然科学基金资助课题(2016A030313601) （2016A030313601）
广东省广州市科技局科技计划项目资助课题(201510010076) （201510010076）
