吉林大学学报（医学版）2017，Vol.43Issue(3)：479-484,6.DOI:10.13481/j.1671-587x.20170304
亚抑菌浓度亚胺培南对MRSA体外增殖及毒力相关基因mRNA表达水平的影响
Effects of sub-inhibitory concentration of imipenem on proliferation in vitro and mRNA expression levels of MRSA virulence related genes
摘要
Abstract
Objective:To explore the effect of sub-inhibitory concentration of imipenem on the bio-activities of methicillin resistant Staphylococcus aureus(MRSA) and illuminate the inhibitory effects of carbapenem antibioty on the activity of MRSA and their mechanisms,and to provide the basis for using the carbapenem antibiotics in the treatment of MRSA infection.Methods:Five ST239 type of MRSA clinical isolates were selected and were co-cultured with 1/10 and 1/2 minimal inhibitory concentration (MIC) of imipenem for 1.5,6.0 and 12.0 h,which were divided into control group(no imipenem),1/10MIC group(1/10MIC of imipenem),and 1/2MIC group(1/2MIC of imipenem).Fluorescent quantitative real-time PCR method was used to determine the relative mRNA expression levels of virulence-related genes fibronectin A(fnbA),staphylococcal protein A(spa),α-hemolysin(Hla),leukocidin D(lek-D),leukocidin E(lek-E),and enterotoxin A in various groups;Spectrophotometry was used to detect the proliferation activity of MRSA strains in various groups in vitro.Results:After co-culture for 6.0 and 12.0 h,the proliferation activities of 5 trains of MRSA in 1/2MIC group in vitro were significantly decreased compared with control group (P<0.01).The relative mRNA expression levels of 6 virulence-related genes of 5 strains of MRSA in 1/10MIC and 1/2MIC groups were significantly decreased compared with control group(P<0.01).After co-culture for 12.0 h,the mRNA expressions of all the tested virulence-related genes were not found.Conclusion:The sub-inhibitory concentration of imipenem shows obviously inhibitory effect on the mRNA expressions of multiple virulence-related genes of ST 239 type of MRSA strains,and higher concentration of imipenem can suppress the proliferation of MRSA strains in vitro.Imipenem shows the potential value in the treatment of the severe MRSA infected patients.关键词
耐甲氧西林金黄色葡萄球菌/毒力/细胞增殖/亚胺培南/亚抑菌浓度Key words
methicillin resistant Staphylococcus aureus/virulence/cell proliferation/imipenem/sub-inhibitory concentration分类
医药卫生引用本文复制引用
王俊瑞,段美庆,额尔德木图,孙鹏,魏常梅,韩艳秋..亚抑菌浓度亚胺培南对MRSA体外增殖及毒力相关基因mRNA表达水平的影响[J].吉林大学学报（医学版）,2017,43(3):479-484,6.基金项目
国家自然科学基金资助课题(81260244,81660352) （81260244,81660352）
内蒙古自治区科技厅科技计划资助课题(20140144) （20140144）
内蒙古自治区卫计委科研项目资助课题(201303061) （201303061）
内蒙古自治区科技厅自然科学基金面上项目资助课题(2016MS0829) （2016MS0829）
