吉林大学学报（医学版）2017，Vol.43Issue(3)：485-490,6.DOI:10.13481/j.1671-587x.20170305
红景天苷对TNF-α诱导的人类风湿关节炎成纤维样滑膜细胞增殖的抑制作用及其意义
Inhibitory effect of salidroside on proliferation of HFLS-RA induced by TNF-α and its significance
摘要
Abstract
Objective:To study the inhibitory effect of salidroside on the proliferation of fibroblast-like synoviocytes with rheumatoid arthritis in human(HFLS-RA) induced by tomor necrossi factor-α(TNF-α),and to clarify the molecular mechanism of its control effect on rheumatoid arthritis(RA).Methods:The HFLS-RA were cultured in vitro,then treated with TNF-α and different concentrations of salidroside.The cells were divided into normal control group(0 μg·L-1TNF-α),model control group(10.0 μg·L-1TNF-α)and 12.5,25.0,50.0,and 100.0 μmol·L-1 salidroside groups(10.0 μg·L-1TNF-α+salidroside).The proliferation activity was detected by MTT mehthod;the expression levels of β-catenin,matrix metalloproteinase-7(MMP-7),and Cyclin-D1 in supernatant of the cells were detected by ELISA method;the expression level of β-catenin protein in cells was detected by Western blotting method.Results:Compared with normal control group,the proliferation activity of the HFLS-RA in model control group was significantly increased (P<0.05);compared with model control group,the proliferation activities of the HFLS-RA in 12.5 and 25.0 μmol·L-1 salidroside groups were decreased but there were no significant differences(P>0.05),and the proliferation activities of the HFLS-RA in 50.0 and 100.0 μmol·L-1 salidroside groups were significantly decreased (P<0.05).Compared with normal control group,the expression levels of β-catenin,MMP-7,and Cyclin-D1 in the supernatant of the cells in model control group were increased(P<0.05).Compared with model control group,the expression levels of β-catenin,MMP-7,and Cyclin-D1 in the supernatant of the cells in 12.5 and 25.0 μmol·L-1 salidroside groups were decreased,but there were no significant differences(P>0.05);the expression levels of β-catenin,MMP-7,and Cyclin-D1 in the supernatant of the cells in 50.0 and 100.0 μmol·L-1 salidroside groups were decreased(P<0.05).The Western blotting results showed that the expression level of β-catenin protein in the cell in model control group was higher than that in normal control group(P<0.01);the expression levels of β-catenin protein in the cells in 12.5 and 25.0 μmol·L-1 salidroside groups were lower than that in model control group,but there were no significant differences(P>0.05);the expression levels of β-catenin protein in the cells in 50.0 and 100.0 μmol·L-1 salidroside groups were lower than that in model control group(P<0.05).Conclusion:Salidroside could inhibit the proliferation of HFLS-RA,and its control effect might be related to the regulation of Wnt/β-catenin single pathway.关键词
类风湿关节炎/红景天苷/成纤维样滑膜细胞/肿瘤坏死因子α/Wnt/β-catenin信号通路Key words
rheumatoid arthritis/salidroside/fibroblast-like synoviocytes/tumor necrosis factor alpha/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
郑洋洋,代东雪,潘志,唐成芳,王颖航..红景天苷对TNF-α诱导的人类风湿关节炎成纤维样滑膜细胞增殖的抑制作用及其意义[J].吉林大学学报（医学版）,2017,43(3):485-490,6.基金项目
国家自然科学基金资助课题(81273651,81374025) （81273651,81374025）
吉林省中医药管理局科研项目资助课题(2014-ZC44) （2014-ZC44）
