吉林大学学报（医学版）2017，Vol.43Issue(3)：507-511,5.DOI:10.13481/j.1671-587x.20170309
重组MUC1-MBP融合蛋白联合R848对小鼠T细胞免疫活性的诱导作用
Inductive effect of recombinant MUC1-MBP fusion protein combined with R848 on immune activity of T cells in mice
摘要
Abstract
Objective:To study the inductive effect of recombinant MUC1-MBP fusion protein combined with R848 on the immune activity of T cells in the mice,and to provide experimental basis for searching the suitable adjuvants of recombinant MUC1-MBP fusion protein.Methods:A total of 21 C57BL/6 mice were randomly divided into control group(treat with normal saline),MUC1-MBP+R848 group (treated with MUC1-MBP+R848),and MUC1-MBP+baillus calmette guerin(BCG) group(treated with MUC1-MBP+BCG)(n=7).After immunized for 4-7 d,the spleen tissue was taken and the spleen indexes of the mice in various groups were measured;the stimmulus index(SI) of the mice was detected by lymphocyte proliferation response;the levels of tumor necrosis factor-γ(TNF-γ) and interleukin-4(IL-4) were detected by ELISA method;the proprotions of T lymphocyte subsets in spleen cells were analyzed by flow cytometry.Results:Compared with control group,the spleen indexes,SI,and TNF-γ levels of the mice in MUC1-MBP+ R848 and MUC1-MBP+BCG groups were significantly increased (P<0.05 or P<0.01);the indexes metioned above of the mice in MUC1-MBP+ R848 were higher than those in MUC1-MBP+BCG group;the IL-4 levels had no significant difference(P>0.05).Compared with control group,the proprotions of CD3+T,CD4+T,and CD8+ T cells of the mice in MUC1-MBP+ R848 group and MUC1-MBP+BCG group were significantly increased(P<0.05 or P<0.01).Conclusion:Both MUC1-MBP fusion protein combined with R848 and BCG can induce the Th1 type of immune activity in the mice,and R848 is the potential candidate adjuvant for MUC1-MBP.关键词
MUC1-MBP/R848/肿瘤疫苗/佐剂Key words
MUC1-MBP/R848/cancer vaccine/adjuvant分类
生物科学引用本文复制引用
孙敏英,刘果木,接晶,谢飞,翟瑞萍,陈潭秀,袁红艳,台桂香..重组MUC1-MBP融合蛋白联合R848对小鼠T细胞免疫活性的诱导作用[J].吉林大学学报（医学版）,2017,43(3):507-511,5.基金项目
吉林省科技厅科技攻关双十工程项目资助课题(20140201012YY) （20140201012YY）
吉林省长春市科技局科技项目资助课题(201622010100025) （201622010100025）
