吉林大学学报（医学版）2017，Vol.43Issue(3)：527-531,5.DOI:10.13481/j.1671-587x.20170313
鹿茸多肽-胶原蛋白/壳聚糖复合材料对家兔下颌骨缺损愈合的促进作用及其机制
Promotive effect of velvet antler polypeptide-collagen/chitosan composite materials on fracture healing of mandibular defect of rabbits and its mechanism
摘要
Abstract
Objective:To prepare the velvet antler polypeptide-collagen/chitosan composite materials,and to investigate its promotive effect on cicatrization of mandibular defect and possible mechanism.Methods:The collagen and chitosan solution were mixed.The composite material was prepared by glutaraldehyde crosslinking method.The microstructure of the composite material was observed by transmission electron microscope (SEM).The unilateral mandibular defect models of 36 rabbits were established.The rabbits were divided into experiment and control groups,and each group was divided into 4-,8-and 12-week subgroups,and there were 6 rabbits in each sub group.The rabbits in experiment group were implanted with velvet antler polypeptide-collagen /chitosan composite materials and the rabbits in control group were treated.4,8 and 12 weeks after operation,the histology of bone defect and peripheral nerve reconstruction of the rabbit models were detected by CT;the expression of vascular endothelial growth factor (VEGF) in bone tissue of the rabbits was detected by immunohistochemistry;the ultrastructure of bone defect was observed by SEM.Results:The structure of composite materials had layered folds and the inner diameter of the stent became larger and mainly dominated by sheet structure,which was the ideal structure of biological materials.4 weeks after operation,the new bone was formatted in experiment group,most of the new bone like-tissue materials were degraded,and the VEGF expression showed an increasing trend;8 weeks after operation,the trabecular bone in the bone defect of the rabbits in experiment group was increased obviously and the expression of VEGF was decreased.12 weeks after operation,the new bone formation and the density in experiment group was consistent with the normal tissue,and the expression level of VEGF returned to normal.At each the point after operation,the degree of bone defect healing and bone formation rate in experiment group were obviously prior to control group.Conclusion:Velvet antler polypeptide-collagen /chitosan composite material has the promotive effect on the fracture healing of mandibular defect of the rabbits and its possible mechanism may be related to promoting the expression of VEGF.关键词
鹿茸多肽/下颌骨缺损/血管内皮生长因子/骨组织工程Key words
velvet antler polypeptide/mandibular defect/vascular endothelial growth factor/bone tissue engineering分类
生物科学引用本文复制引用
朱文赫,钟秀宏,张巍,徐俊杰,李妍,沈楠,张红,吕士杰..鹿茸多肽-胶原蛋白/壳聚糖复合材料对家兔下颌骨缺损愈合的促进作用及其机制[J].吉林大学学报（医学版）,2017,43(3):527-531,5.基金项目
吉林省科技厅科技发展计划项目资助课题(20120941,20140204002YY) （20120941,20140204002YY）
吉林省卫计委青年科研基金资助课题(2015Q041) （2015Q041）
吉林省吉林市科技局科技计划项目资助课题(20156428) （20156428）
