吉林大学学报（医学版）2017，Vol.43Issue(3)：593-599,7.DOI:10.13481/j.1671-587x.20170325
复方总黄酮聚乳酸-羟基乙酸共聚物微囊的制备及体外释药特性
Preparation of compound TF-PLGA microcapsule and its drug release characteristics in vitro
摘要
Abstract
Objective:To use polylactic-co-glycolic acid(PLGA) as vector material to prepare the compound total flavonoids of Rhizoma Drynariae(TFRD) and total flavonoids of Chrysanthemum(TFC) sustained release microcapsule TF-PLGA microcapsules,and to investigate the the best preparation technique of TF-PLGA microcapsules and their sustained release characteristics in vitro.Methods:The TF-PLGA microcapsules were prepared with TFRD,TFC,and PLGA by emulsifying-solvent evaporation technique under certain conditions.With the encapsulation efficiency(EE) as the evaluation indicator,the optimal formulation was verified by single factor experiment and orthogonal design;the general morphology,the particle size and distribution of the microcapsules were observed by light microscope(LM) and scanning electron microscope(SEM);the cumulative drug release rate of TF-PLGA microcapsules was detected by constant temperature commotion method in vitro and the release curves of the TF-PLGA were drawn.Results:The optimal prescription was as follows:the concentration of PLGA was 140 g·L-1,oil phase volume was 1.4 mL,emulsifying speed was 900 r·min-1,emulsifying time was 5 min,the average EE was(83.89±2.30)%,and the average drug loading rate(DL) was(5.90±0.07)%.The LM and SEM resluts showed that the TF-PLGA microcapsules presented as round ball,the average particle size was(44.34±14.68)μm,and the distribution was relatively narrow.The drug release in vitro results showed that the initial drug release rate(24 h)was about 40%,and the cumulative drug release rate was over 90% after 50 d.Conclusion:The TF-PLGA sustained release microcapslue has better drug-loaded and sustained-release effects with simple preparation technique and better repeatability.关键词
骨碎补总黄酮/野菊花总黄酮/聚乳酸-羟基乙酸/微囊/缓释Key words
total flavonoids of Rhizoma Drynariae/total flavonoids of Chrysanthemum/polylactic-co-glycolic acid/microcapsules/slow-release分类
医药卫生引用本文复制引用
陈楠楠,马宁,车鸿泽,焦鹏,陈飞,张莉..复方总黄酮聚乳酸-羟基乙酸共聚物微囊的制备及体外释药特性[J].吉林大学学报（医学版）,2017,43(3):593-599,7.基金项目
吉林省中医药管理局科研项目资助课题(2014-Q48) （2014-Q48）
