草业学报2017，Vol.26Issue(5)：62-69,8.DOI:10.11686/cyxb2016252
青蒿素对牧草的化感作用
Allelopathic effects of artemisinin on pasture grasses
摘要
Abstract
Artemisiaannua,which grows in many parts of Chongqing,releases artemisinin,which can inhibit the growth of surrounding plants.To identify pasture grasses that can grow in areas with abundant A.annua, we conducted a pot experiment to study the effects of artemisinin on grass growth.We evaluated the growth, physiology,antioxidant enzyme activity,and nutrient uptake of three pasture grasses (Loliumperenne,Trifo-liumrepens,and Viciavillosa)growing in soil with artemisinin at different concentrations (0 ,control;6 ,12 , 24,36 mg/kg soil).Artemisinin significantly affected the growth of all three pasture grasses,and the effects differed depending on the artemisinin concentration and the grass species.At 6 mg/kg,artemisinin led to sig-nificant decreases in growth indexes and the degree of inhibition increased with increasing artemisinin concen-trations.Compared with their respective controls,L.perenne,T.repens,and V.villosaplants grown with artemisinin at 36 mg/kg showed 76.83%,90.10%,and 65.09% decreases in biomass,respectively,and 47.82%,38.76% and 44.49% decreases in plant height,respectively.Artemisinin had a negative allelopathic effect on all three pasture grasses,with the strongest effect onT.repens,followed byL.perenneand thenV.villosa,indicating thatV.villosawas the least sensitive to artemisinin.Therefore,V.villosamay be the most adaptable of the three tested grasses to growth in areas of Chongqing with abundant A.annua.In addition,ar-temisinin significantly reduced the chlorophyll content,root vigor,nitrate reductase activity,photosynthetic rate,root nutrient uptake,and nitrogen assimilation in all of the tested grasses.As a result,the uptake of ni-trogen,phosphorus,and potassium by pasture grasses was decreased by more than 80%.The activity of pro-tective oxidases in pasture grasses varied in response to artemisinin,with superoxide dismutase showing in-creased activity,peroxidase showing decreased activity,and catalase showing no significant change in activity. However,artemisinin greatly increased the malondialdehyde content in all three pasture grasses,suggesting that more oxyradicals were produced or less oxyradicals were eliminated,leading to cell membrane damage.关键词
青蒿素/牧草/化感作用Key words
artemisinin/pasture grass/allelopathy effect引用本文复制引用
魏立本,夏志林,殷洁,袁玲..青蒿素对牧草的化感作用[J].草业学报,2017,26(5):62-69,8.基金项目
科技部"973"课题(2013CB127405)资助. （2013CB127405）
