草业学报2017，Vol.26Issue(5)：100-108,9.DOI:10.11686/cyxb2016429
外源无机盐与硫胺素对枝叶去除后 紫花苜蓿的再生性影响
Effects of exogenous inorganic salts and thiamine on the regrowth of defoliated alfalfa
摘要
Abstract
Grazing integrates multiple processes including bruising,defoliation,and the deposition of saliva, which may affect the regrowth of forage grasses.In this study,alfalfa plants defoliated at different intensities were treated with exogenous inorganic salts and with thiamine (treatment groups ),and deionized water and sheep saliva (control groups).The aim of this study was to determine whether a herbivorous animal's (sheep) saliva,especially its components (inorganic salts and thiamine)positively affect alfalfa regrowth.We also ana-lyzed how the defoliation and sheep saliva interaction induced compensatory regrowth,and evaluated the mor-phological and physiological differentiation of defoliated alfalfa after treatment with inorganic salts and thia-mine.Our results showed that,compared with alfalfa in the defoliation-only treatment,that in the saliva+de-foliation treatment showed significantly better regrowth due to an effective ingredient in saliva (thiamine ). Compared with defoliated alfalfa treated with exogenous inorganic salts,that treated with thiamine grew bet-ter,as indicated by the significantly greater aboveground biomass,4.3-7.9-times greater stem/leaf ratio,and 1.56-times higher total tiller number at the 80% defoliation level.The physiological characteristics of defolia-ted alfalfa did not vary widely among treatments.In all treatments,the chlorophyll content of defoliated alfalfa was close to 49 .3 mg/g.At the 20% defoliation level,alfalfa plants treated with sheep saliva and with water showed significantly increased soluble sugars contents in the roots,compared with those in the inorganic salts and thiamine treatments.Treatment with inorganic salts led to a progressive increase in peroxidase activity as the defoliation intensity increased,while treatment with thiamine did not affect peroxidase activity.These re-sults increase our understanding of the functional mechanism of the alfalfa response to herbivore grazing.These results provide a reference for improving the status of artificial grassland and for improving the agricultural effi-ciency of cultivated forage grasses.The results of this study provide theoretical support for the development of ecologically sound grazing regimes.关键词
枝叶去除/紫花苜蓿/无机盐/硫胺素/再生性Key words
defoliation/alfalfa/inorganic salt/thiamin/regrowth引用本文复制引用
赵威,李亚鸽,王馨,李涛..外源无机盐与硫胺素对枝叶去除后 紫花苜蓿的再生性影响[J].草业学报,2017,26(5):100-108,9.基金项目
NSFC-河南人才培养联合基金(U1304306),中国科学院战略性先导科技专项子课题(XDA05050400)和河南科技大学学科提升振兴A计划项目资助. （U1304306）
