川北医学院学报2017，Vol.32Issue(2)：155-159,5.DOI:10.3969/j.issn.1005-3697.2017.02.001
不同剂量分割模式照射Eca109细胞TNF和SODD的表达
Expression of TNF and SODD in Eca109 cells under different dose fractionation mode irradiation
摘要
Abstract
Objective:Hope to provide some theoretical basis for unconventional radiotherapy,we observe and compare expression of apoptosis related genes (TNF and SODD)between groups by simulating different dose fractionation on esophageal cancer cells Eca109.Methods:Design the dose fractionation mode with the same BED.Irradiation is exponential growth phase Eca109 of cells.Detect TNF mRNA and SODD mRNA,expression in each group with RT-PCR on the first day after simulation irradiation.Detect TNF protein and SODD proteins,expression in each group with Western Blot on the first day after simulation irradiation.Results:Mean differences of the TNF mRNA's and protein's expression quantity in each group were compared,all were statistically significant;Mean differences of the SODD mRNA's and protein's expression quantity in each group were compared,all were statistically significant.Conclusion:Among the simulation irradiation groups,the hyperfractionated group's TNF mRNA's expression was the highest,so was SODD mRNA.It suggests that a fight should exist between promoting apoptotic genes and anti-apoptotic gene in the process of induced tumor cells' dying.In the analog irradiation groups,both TNF protein and SODD protein suffered massive reduction.It suggests that injury of other organelles except DNA can not be ignored,because their functional status may affect the synthesis of protein.关键词
剂量分割/Eca109/肿瘤坏死因子/死亡结构域沉默子Key words
Dose fractionation/Eca109/Tumor necrosis factor/Silencer of death domains分类
医药卫生引用本文复制引用
蔡红伟,马代远,李贤富,周进伟,蒲宇,董灵,谭榜宪..不同剂量分割模式照射Eca109细胞TNF和SODD的表达[J].川北医学院学报,2017,32(2):155-159,5.基金项目
中央财政支持地方高校发展专项资金(2010-2012年) （2010-2012年）
四川省教育厅重点课题(11ZA191) （11ZA191）
南充市科技支撑计划项目(11A0149) （11A0149）
