川北医学院学报2017，Vol.32Issue(2)：172-174,3.DOI:10.3969/j.issn.1005-3697.2017.02.005
转移相关基因2对喉癌细胞系Hep-2的增殖、迁移和侵袭的影响
Effect of metastasis associated gene 2 on proliferation,migration and invasion of laryngeal carcinoma cell line Hep-2
摘要
Abstract
Objective:To investigate the effect of metastasis associated gene 2 (MTA2) on the proliferation,migration and invasion of laryngeal carcinoma cell line Hep-2.Methods:MTA2 gene expression was down regulated by small interfering RNA (siRNA-MTA2) of MTA2 gene,and the transfection was detected by RT-PCR and Transwell.As the experimental group,the control group was transfected with negative control (sicontrol),and the effect of MTA2 on the proliferation,migration and invasion of Hep-2 was detected by MTT,cell scratch test and Transwell assay.Results:the expression level of MTA2mRNA and protein in the experimental group was significantly lower than that in the control group (P<0.05).The cell proliferation ability of the experimental group was significantly lower than that of the control group (P<0.05) when cultured in 7d and 9D,The cell migration ability and invasion ability of the experimental group were significantly lower than those of the control group (P<0.05).Conclusion: MTA2 is closely related to the proliferation,migration and invasion of Hep-2 cell line,which is expected to be a target for the treatment of laryngeal cancer.关键词
MTA2/喉癌细胞/Hep-2/增殖/迁移Key words
MTA2/Laryngeal cancer cell/Hep-2/Proliferation/Migration分类
医药卫生引用本文复制引用
马鹏,冯俊,杜经纬..转移相关基因2对喉癌细胞系Hep-2的增殖、迁移和侵袭的影响[J].川北医学院学报,2017,32(2):172-174,3.基金项目
四川省教育厅项目(13ZB0241) （13ZB0241）
川北医学院博士科研启动项目(CBY-QD-08) （CBY-QD-08）
