重庆医学2017，Vol.46Issue(14)：1889-1891,1896,4.DOI:10.3969/j.issn.1671-8348.2017.14.006
维生素E联合红景天对低氧复合运动小鼠骨骼肌保护作用的研究
The protective effects of Rhodiola rosea combined with vitamin E on the skeletalmuscle through hypoxia combined with exercise training in mice
庞巨涛 1张新虎 1孙建华 1周连军 1刘斌 1李凤国 1梁园园2
作者信息
- 1. 哈励逊国际和平医院骨科,河北衡水 053000
- 2. 武警辽宁省总队医院骨科,辽宁沈阳 110034
- 折叠
摘要
Abstract
Objective To study the protective effects of vitamin E combined with Rhodiola rosea on the injury in mice skeletal muscle through hypoxia with exercise training and research the mechanism of action.Methods Forty ICR mice were randomly divided into control group and observation group,20 mice in each group.The control group was given Vitamin E (40 mg·kg-1·d-1),and the observation group was given Vitamin E (40 mg·kg-1·d-1)combined with Rhodiola rosea (20 g·kg-1·d-1);the rats in the two groups were treated with hypoxia (11.3%) for 4 weeks (5 ℃,15 m/min,60 min/d) after 15-day drug using.After the last training,all the mice were euthanized and then detected the gene of Bax and Bcl-2 mRNA through the RT-PCR in the skeletal muscle and the activity of GSH-PX,SOD,ROS,the concentration of MDA in the skeletal muscle.During the period (pretrain and 1,2,4 weeks) of hypoxia combined with exercise training,the CK,LDH in the serum were detected.Results The expression of CK and LDH in the observation group were lower than those of the control group (P<0.05).The activity of GSH-PX and SOD in the skeletal muscle of the observation group was significantly higher than that of the control group (P<0.05),while the ROS and MDA were lower than those of the control group(P<0.05);the expression of Bax mRNA and Bax/Bcl-2 of the observation group was significantly lower than that of the control group(P<0.05).While the expression of Bcl-2 mRNA was significantly higher than that of the control group(P<0.05).Conclusion Rhodiola rosea combined with Vitamin E can inhibit the expression of apoptotic genes by correcting the oxidation and hypoxia imbalance under hypoxia.关键词
红景天/维生素E/肌,骨骼/低氧/运动/氧化/凋亡Key words
Rhodiola sacra/vitamin E/muscle/skeletal/hypoxia/exercise training/oxidize/apoptosis分类
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庞巨涛,张新虎,孙建华,周连军,刘斌,李凤国,梁园园..维生素E联合红景天对低氧复合运动小鼠骨骼肌保护作用的研究[J].重庆医学,2017,46(14):1889-1891,1896,4.
