重庆医学2017，Vol.46Issue(14)：1892-1896,5.DOI:10.3969/j.issn.1671-8348.2017.14.007
CD4+T细胞中IL-18激活的NF-κB信号通路与PBC发病机制的相关性研究
Correlation analysis of NF-κB signaling pathway activated by IL-18 in CD4+ T cells and the pathogenesis of PBC
摘要
Abstract
Objective To explore the correlation between NF-κB signaling pathways activated by IL-18 in CD4+ T cells and the pathogenesis of PBC.Methods We detected the expression of IL-18 mRNA in PBMCs,IL-18 level in plasma,receptor IL-18R on surface of CD4+ T cell,proliferation rate of CD4+T cell and its NF-κB signaling pathway protein IκBα and NF-κB p65 by qRT-PCR,ELISA,flow cytometry,MACS and Western blot on 32 cases of patients with PBC (PBC group) and 32 healthy people (control group) in Guizhou provincial people′s hospital.Results The level of IL-18 in PBC group was significantly higher than that in control group (P<0.05).The relative expression of IL-18 mRNA in PBC group was significantly higher than that in control group (P<0.05).The percentage of CD4+T cells expressing IL-18Rα in PBC group was higher than that in control group (P<0.05).The proliferation rate of CD4+T cells stimulated by IL-18 in PBC group was significantly higher than that in healthy control group (P<0.01).The relative expression levels of NF-κB p65 protein were up-regulated in IL-18,and the expression of IκBα protein in each group was significantly increased,especially in PBC group (P<0.01).Conclusion IL-18 can activate NF-κB signal pathway in CD4+ T cells and participate in the pathogenesis of primary biliary cirrhosis.关键词
肝硬化,胆汁性/T淋巴细胞/抗原,CD4/白细胞介素18/原发性胆汁性肝硬化/NF-κB信号通路Key words
liver cirrhosis/biliary/T-lymphocytes/antigens/CD4/interleukin-18/primary biliary cirrhosis/nuclear factor-kappa signaling pathway分类
医药卫生引用本文复制引用
赵二川,李红梅,任智晶,何宇晴,王明珠,叶震旋,周雯婧,张华..CD4+T细胞中IL-18激活的NF-κB信号通路与PBC发病机制的相关性研究[J].重庆医学,2017,46(14):1892-1896,5.基金项目
贵州省科学技术厅项目(黔科合LH字[2014]7008号). （黔科合LH字[2014]7008号）
