重庆医学2017，Vol.46Issue(14)：1941-1943,1946,4.DOI:10.3969/j.issn.1671-8348.2017.14.023
人心房肌细胞分离方法的改良研究
An improved method for isolation of human atrial cardiomyocytes
摘要
Abstract
Objective To study an improved isolated method of single human atrial myocytes.Methods Enzyme digestion method was used to isolate single myocytes from human atrial and whole-cell patch clamp technique was used to record small conductance calcium activated potassium current.Results This method obtained a large number of atrial myocytes.The total amount of atrial myocytes in SR group was 320±30 while AF group was 230±20 and the difference was statistically significant(P<0.01).In this study,a large number of simple and striated single atrial myocytes were obtained,and a typical small-conductance calcium-activated potassium channel current was recorded on the isolated atrial myocytes.Conclusion The established isolated method is simple,stable and effective.We can acquire a large amount of single atrial myocytes with good quality.关键词
电学/肌细胞,心脏/人心房肌/分离方法/膜片钳/离子通道Key words
electricity/myocytes/cardiac/human atria myocytes/isolated method/patch-clamp technique/ionic channel分类
医药卫生引用本文复制引用
王笑宇,范忠才,周伟,余奕言,周涛,李妙龄..人心房肌细胞分离方法的改良研究[J].重庆医学,2017,46(14):1941-1943,1946,4.基金项目
国家自然科学基金资助项目(81470022) （81470022）
四川省教育厅重点项目(16ZA0192) （16ZA0192）
泸州市-泸州医学院联合项目(20140975). （20140975）
