重庆医学2017，Vol.46Issue(15)：2017-2020,4.DOI:10.3969/j.issn.1671-8348.2017.15.001
阻断胃泌素受体对胃癌细胞增殖和凋亡及其信号通路的影响
Influence of blocking gastrin receptor on the proliferation and apoptosis and expression of keyproteins in related pathway of gastric cancer cell
摘要
Abstract
Objective To investigate the effects of blocking gastrin receptor on the proliferation,apoptosis and expression of key proteins in the related pathway in gastric cancer cell lines.Methods In the experimental group,the gastric cancer cell lines SGC-7901 and AGS cells were treated with 5 mmol/L proglumide,a kind of a gastrin receptor antagonist.And the normal cultured gastric cancer cells SGC-7901 and AGS were used in control group.The growth of each group was detected by MTT assay;the cell growth curve was drawn by flow cytometry;the cell cycle of each group was detected by flow cytometry.Annexin V-FITC/PI double staining was used to detect the cell growth of apoptosis.The relative mRNA expression of β-catenin,nuclear factor-P65,mammalian target of rapamycin and glycogen synthase kinase 3 beta in Wnt,NF-κB and PI3K-AKT-MTOR pathways were detected by RT-qPCR.The expression of β-catenin protein was detected by Western blotting.Results After treatment with proglumide,the growth of the cells in the experimental group was lower than that in the control group;and the proportion of S phase cells in the cell cycle was also lower than that in the control group,but the proportion of cells in G0/G1 phase was higher than that in the control group(P<0.05).The percentage of apoptotic cells was also increased after treatment with proglumide(P<0.05).Furthermore,proglumide treatment significantly reduced the expression of β-catenin at both mRNA and protein levels(P<0.05).Conclusion Blocking gastrin receptor can down-regulate the expression of β-catenin,inhibit the cell proliferation and promote the cell apoptosis in gastric cancer cells.关键词
胃肿瘤/胃泌素类/细胞凋亡/细胞增殖/丙谷胺/β-cateninKey words
stomach neoplasms/gastrins/apoptosis/cell proliferation/proglumide/β-catenin分类
医药卫生引用本文复制引用
袁航,汪闯,王琴容,赵艳,李雅洁,龙妮娅,周建奖..阻断胃泌素受体对胃癌细胞增殖和凋亡及其信号通路的影响[J].重庆医学,2017,46(15):2017-2020,4.基金项目
国家自然科学基金资助项目(81260303,31560326) （81260303,31560326）
贵州省科技合作计划项目(黔科合LH字[2015]7360). （黔科合LH字[2015]7360）
