重庆医学2017，Vol.46Issue(15)：2034-2036,3.DOI:10.3969/j.issn.1671-8348.2017.15.006
胃促生长素对人主动脉平滑肌细胞增殖及线粒体融合蛋白2表达的影响
Effect of ghrelin on proliferation and mitofusin-2 expression of human aortic smooth muscle cells
何永铭 1宋明宝 1胡建波 1张源萍 1李佑美1
作者信息
- 1. 第三军医大学新桥医院心血管内科,重庆 400037
- 折叠
摘要
Abstract
Objective To investigate the effects of ghrelin on proliferation of vascular smooth muscle cells(VSMC)and the expression of mitochondrial fusion 2(Mfn-2)in cultured human aortic smooth muscle cells(HASMCs).Methods HASMCs were cultured in vitro,treated with different concentrations(10-9,10-8,10-7,10-6,10-5 mol/L)ghrelin or 10-6 mol/L ghrelin for different time(0,6,12,18,24 h).Subconfluent HASMCs at passage 4-6 were used in experiments.MTT essay was used to investigate the effect on proliferation of HASMCs.RT-PCR and Western blot were used to analyse the expression of Mfn-2.Results 10-7-10-5 mol/L ghrelin inhibited the proliferation of HASMCs,and the inhibitory effect of concentration of 10-6 mol/L was the most obvious(P<0.01).Ghrelin inhibited the proliferation of HASMCs in 6-24 h,and it reached the peak at 24 h(P<0.01).10-6 mol/L ghrelin significantly increased the expression of Mfn-2 mRNA and protein(P<0.01).The up-regulation of 10-6 mol/L ghrelin on Mfn-2 mRNA and protein expression reached the peak at 18 h(P<0.01).Conclusion Ghrelin might inhibit the proliferation of HASMC by up-regulating the expression of Mfn-2.关键词
胃促生长素/肌,平滑,血管/主动脉/人主动脉平滑肌细胞/增殖/线粒体融合蛋白-2Key words
ghrelin/muscle/smooth/vascular/aorta/human aortic smooth muscle cells/proliferation/mitofusin 2分类
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何永铭,宋明宝,胡建波,张源萍,李佑美..胃促生长素对人主动脉平滑肌细胞增殖及线粒体融合蛋白2表达的影响[J].重庆医学,2017,46(15):2034-2036,3.
