动物营养学报2017，Vol.29Issue(5)：1527-1534,8.DOI:10.3969/j.issn.1006⁃267x.2017.05.010
不同相对湿度对间歇性26℃环境下肉鸡盲肠菌群多样性的影响
Effects of Different Relative Humidity on Cecal Microflora Diversity of Broilers under Intermittent 26 ℃ Environment
摘要
Abstract
This study was carried out to investigate the effects of different relative humidity ( RH) on cecal mi?croflora diversity of broilers under intermittent 26 ℃ environment. One hundred and eighty 29?day?old Arbor Acres ( AA) broilers were assigned to three environment chambers ( RH were 30%, 60% and 85%, respec?tively) , each chamber contained six cages with ten birds per cage ( five males and five females) , and each cage as a replicate. When broilers were 29 days of age, the temperature of groups was at 26 ℃, regulating the RH to 30%, 60% and 85%, and the temperature and RH of groups were kept six hours each day at 10:00 to 16:00, and broilers were kept at 21 ℃ and 60% RH in the other time. The trial period lasted for 14 days. The effects of RH on bacterial community and diversity in the cecal digesta of broilers at the 7th and 14th day under intermittent 26 ℃ environment were analyzed by using 16S rDNA?based denaturing gradient gel electrophoresis ( DGGE) , combined with the cloning and sequencing of DNA recycled by specificity and generality stripe tap?ping. The results showed as follows: 1) on the 7th day, cecum DGGE bands number ( flora abundance) of broilers in 30% RH group was higher than that in 60% RH group, while in 85% RH group was lower than that in 60% RH group; on the 14th day, cecum DGGE bands number of broilers in 30% and 85% RH groups was higher than that in 60% RH group. 2) Cluster analysis showed that on the 7th day, 85% RH affected the cecal microflora obviously, but on the 14th day, 30% RH did. And as treatment time processing, 30% RH made a greater effect on cecal microflora. 3) The common cecal microbiota of different RH groups under intermittent 26 ℃ environment was Faecalibacterium prausnitzii, and on the 7th day, the specific cecal microbiota in 30%and 85% RH groups was Stomatobaculum longum. In conclusion, at the intermittent 26 ℃ environment, 30%and 85% RH can change the structure and diversity of cecal microflora of broilers, and the effects of RH at dif?ferent processing time are different.关键词
相对湿度/间歇热/肉鸡/盲肠菌群/变形梯度凝胶电泳Key words
relative humidity/intermittent partial heat environment/broilers/cecal microbiota/denaturing gradient gel electrophoresis分类
农业科技引用本文复制引用
彭骞骞,周莹,王雪敏,冯京海,甄龙,张少帅,常玉,石玉祥,张敏红..不同相对湿度对间歇性26℃环境下肉鸡盲肠菌群多样性的影响[J].动物营养学报,2017,29(5):1527-1534,8.基金项目
国家科技支撑计划课题"畜禽健康养殖环境控制关键技术研究与集成"(2012BAD39B02) （2012BAD39B02）
中国农业科学院科技创新团队项目( ASTIP?IAS07) （ ASTIP?IAS07）
