动物营养学报2017，Vol.29Issue(5)：1535-1544,10.DOI:10.3969/j.issn.1006⁃267x.2017.05.011
3~6月龄伊犁马肠道微生物群落多样性的研究
A Study on Intestinal Microbiota Diversity of 3?to 6?Month?Old Yili Horses
摘要
Abstract
The current study was conducted to evaluate the intestinal microbiota diversity of 3?to 6?month?old Yili horses, to reveal the regulation of succession of intestinal micrbiota before weaning, and to provide a theo?retical basis for the researches of growth and intestinal health of young horse. Five 3?month?old Yili horses with the average body weight of (89.75±8.81) kg and the same birth day were selected to conduct a 90?day feeding trial. The feces samples were collected on days 0 (1 day before the experiment), 30, 60 and 90 of the experi?ment, respectively. The microbial genomic total DNA was extracted from each feces sample. Microbiota diver?sity of feces was evaluated using Illumina HiSeq sequencing technology. The results showed as follows: 1 ) twenty feces samples for 5 colts were sequenced, a total of 157665 effective sequences and 1117 average op?erational taxonomic units ( OTUs) were acquired. 2) The alpha diversity indexes ( ACE, Chao1, Shannon and Simpson indices) of feces microbiota showed fluctuated changes with the age increasing, but the changes were not significant among different time points ( P>0.05) . 3) At the phylum level, ten dominant bacteria in feces of colts were Firmicutes, Bacteroidetes, Proteobacteria, Verrucomicrobia, Spirochaetes, Fusobacteria, Teneri?cutes, Actinobacteria, TM7 and Euryarchaeota, and the abundances of Firmicutes, Bacteroidetes, Proteobacte?ria and Verrucomicrobia were higher. At the family level, ten dominant bacteria in feces of colts were Bacil?laceae, Moraxellaceae, Planococcaceae, Carnobacteriaceae, BS11, RFP12, Lactobacillaceae, Ruminococ?caceae, Lachnospiraceae and Porphyromonadaceae. At the genera level, ten dominant bacteria in feces of colts were Acinetobacter, Desemzia, Lactobacillus, Ureibacillus, Paludibacter, Bacillus, Escherichia, Carnobac?terium, Treponema and Mogibacterium. Base on the results, it is concluded that the intestinal microbiota of 3?to 6?month?old Yili horses can be accurately classified using Illumina HiSeq sequencing technology. The Firmi?cutes, Bacteroidetes, Proteobacteria, Verrucomicrobia were the dominant bacteria in intestine of 3?to 6?month?old Yili horses.关键词
伊犁马/IlluminaHiSeq测序技术/肠道微生物群落/多样性Key words
Yili horses/Illumina HiSeq sequencing technology/intestinal microbiota/diversity分类
农业科技引用本文复制引用
李晓斌,赵国栋,刘振,吴婷婷,何周瑞,邓海峰,杨开伦..3~6月龄伊犁马肠道微生物群落多样性的研究[J].动物营养学报,2017,29(5):1535-1544,10.基金项目
"十二五"农村领域国家科技计划课题(2012BAD45B01) （2012BAD45B01）
