动物营养学报2017，Vol.29Issue(5)：1552-1559,8.DOI:10.3969/j.issn.1006⁃267x.2017.05.013
阴外动脉灌注乳脂前体物对奶牛产奶量及乳腺内短链脂肪酸摄取规律的影响
Effects of Infusing Milk Fat Precursors to External Pudic Artery on Milk Yield and Uptake Rule of Short?Chain Fatty Acids in Mammary Gland of Dairy Cows
摘要
Abstract
This experiment was conducted to investigate the effects of infusing milk fat precursors to external pudic artery on milk yield and uptake rule of short?chain fatty acids in mammary gland of dairy cows. Eight healthy Holstein cows were divided into two groups with four cows per group. A 2×2 cross?over experimental design was adopted. Cows in the two groups were fed diets with two kinds of roughages: corn stover ( CS group) and alfalfa ( MF group) , and the diet concentrate to roughage ratio was 45:55. The experiment was di?vided into two stages, and each stage lasted for 20 days, including 14 days of preliminary feeding period, 3 days of carrier infusion phase and 3 days of milk fat precursors infusion phase. During carrier infusion period, MF group and CS group received carrier infusion, and were named MFC1 group and CSC group, respectively;during milk fat precursors infusion period, MF group continued to accept carrier infusion ( MFC2 group), while CS group accepted milk fat precursors infusion ( CSF group) . Milk yield, dry matter intake and milk composition were determined. Plasma in caudal artery and internal mammary vein was collected to determine short?chain fatty acid concentrations and fatty acid proportions. The results showed as follows:1) before milk fat precursors infusion, CSC group had significantly lower milk yield, 4% fat corrected milk ( FCM) yield, milk fat yield, milk protein yield and milk fat producing efficiency than MFC1 group (P<0.05), and short?chain fatty acid concentration and short?chain fatty acid to long?chain fatty acid rate in plasma of caudal artery in CSC group tended to be higher than those in MFC1 group (0.05≤P<0.10). 2) After milk fat precursors in?fusion, milk fat producing efficiency and milk protein percentage in CSF group were significantly higher than those in CSC group ( P<0.05) , and CSF group still below in MFC2 group in milk yield, 4% FCM yield, milk fat percentage and milk protein percentage, but no significant differences was found between groups ( P>0.05); CSF group was significantly higher than MFC2 group in milk fat producing efficiency (P<0.05). 3) After milk fat precursors infusion, the concentrations of acetic acid, propionic acid and short?chain fatty acids in plasma of caudal artery in CSF group were significantly higher than those in CSC group ( P<0.05) , and the concentrations of acetic acid, butyric acid and short?chain fatty acids in CSF group was significantly higher than those in MFC2 group ( P<0.05) , but short?chain fatty acids to long?chain fatty acids rate in CSC group tended to be lower than that in MFC2 group (0.05≤P<0.10). 4) Except that artery?vein difference of butyric acid in CSF group tended to be higher than that in CSC group (0.05≤P<0.10), and there were no significant differ?ences in uptake quantity, uptake rate and artery?vein difference of acetic acid, propionic acid, butyric acid and short?chain fatty acids between CSC group and MFC1 group, CSC group and CSF group, CSF group and MFC2 group ( P>0.05) . The results indicate that dairy cows fed corn stover as roughage infused milk fat pre?cursors can significantly increase the milk yield, milk fat percentage and milk fat yield, and closely achieves the levels in dairy cows fed alfalfa as roughage, especially has producing efficiency of milk fat, but has no sig?nificant effects on uptake quantity, uptake rate of short?chain fatty acids in mammary gland.关键词
乳腺/阴外动脉/乳脂前体物/短链脂肪酸Key words
mammary gland/external pubic artery/milk fat precursor/short⁃chain fatty acid分类
农业科技引用本文复制引用
冯永辉,闫素梅,韩慧娜,生冉,郭晓宇..阴外动脉灌注乳脂前体物对奶牛产奶量及乳腺内短链脂肪酸摄取规律的影响[J].动物营养学报,2017,29(5):1552-1559,8.基金项目
国家奶业"973计划"项目(2011CB1008003) （2011CB1008003）
