福建农业学报2017，Vol.32Issue(3)：258-262,5.DOI:10.19303/j.issn.1008-0384.2017.03.006
鳗鲡幼苗种类的DNA指纹鉴定
Molecular Identification of Juvenile Eels by DNA Fingerprints
摘要
Abstract
Accurate species identification is crucial for eel aquaculture,especially when increasing taxa of the fish were raised in the eel farms in China.This study applied a pair of specific primers from the conservative fragment of mtDNA COI gene in Anguilla to amplify 109 DNA samples extracted from 21 batches of eel juveniles for taxon identification.It was found that a specific 652bp fragment could be amplified from all of the samples.The BOLD Identification System (Barcoding of Life Data Systems: www.boldsystems.org) was used for sequence similarity search on the COI genes.Seven species,including A.japonica,A.rostrata,A.anguilla,A.marmorata,A.mossambica,A.luzonensis and A.bicolor pacifica,were identified.The cluster analysis showed 7 main clades with high rate of support at the nodes.It suggested that the COI identification method could be satisfactorily employed for eel juveniles identification.Incidentally,during the course of this study a former mistaken identification on a batch of juvenile fish was discovered.Therefore,the DNA barcode-based identification method was highly recommended for implementation to benefit the commercial trade and eel aquaculture.关键词
鳗鲡/幼苗/COI基因/分子鉴定Key words
eel/juveniles/COI/molecular identification分类
生物科学引用本文复制引用
林能锋,陈强,杨金先,许斌福,曾红..鳗鲡幼苗种类的DNA指纹鉴定[J].福建农业学报,2017,32(3):258-262,5.基金项目
福建省科技计划项目--省属公益类科研院所基本科研专项(2015R1019-11) （2015R1019-11）
