福建农业学报2017，Vol.32Issue(3)：263-267,5.DOI:10.19303/j.issn.1008-0384.2017.03.007
草菇VEGF信号通路中vv-SPK基因的结构与表达分析
Structure and Expression Analysis of vv-SPK Gene in VEGF Signaling Pathway of Volvariella volvacea
摘要
Abstract
VEGF signaling pathway regulates cell proliferation,differentiation,migration,stress response,and survival of fungi.A gene,vv-SPK,in the pathway coding SPK protein from the mushroom,Volvariella volvacea,was obtained.A structure analysis showed that the gene was 1 996 bp,including 11 introns,and the open reading frame (ORF) of 1 401 bp encoding 466 amino acids.BLASTP comparison showed vv-SPK sharing a high similarity with the genes from Galerina patagonica,Laccaria bicolor and Laccaria amethystine.RT-qPCR verified the gene expression of SPK protein being associated with the stipe elongation.Since SPK,with its protein kinase characteristics,involves in the cell proliferation pathway in VEGF,the protein was postulated to play a significant role in enhancing the cell division during the stipe development of V.volvacea.There might also be a SPK-mediated signaling pathway that promoted the cell proliferation in V.volvacea.关键词
VEGF通路/草菇/菌柄伸长/促进增殖Key words
VEGF pathway/Volvariella volvacea/stipe elongation/promote proliferation分类
生物科学引用本文复制引用
王健,万芳,李肖,严俊杰,黄李琳,谢宝贵..草菇VEGF信号通路中vv-SPK基因的结构与表达分析[J].福建农业学报,2017,32(3):263-267,5.基金项目
国家重点基础研究发展计划(973 项目)(2014CB138302) （973 项目）
