福建农业学报2017，Vol.32Issue(3)：273-277,5.DOI:10.19303/j.issn.1008-0384.2017.03.009
水晶花烛的无菌播种和组培快繁技术研究
Aseptic Sowing and Rapid Propagation of Anthurium crystallinum
摘要
Abstract
Anthurium crystallinum seedlings were aseptically obtained from seeds for the study.Stem segments with node,leaves and petiole were used as plantlets,and the main factors of callus induction and differentiation as well asthe bud proliferation and seedling rooting during the propagating process were investigated.The results showed that theseed disinfection could be satisfactorily achieved by treating the peeledseeds with 0.1% mercuric chloride for 8min;the optimal callus differentiation of theplantlets was to use the stem segment with node,and cultivated in medium containing 1/3 MS+ 6-BA 1.0 mg·L-1+ 2,4-D 0.3 mg·L-1;the appropriate medium forthe bud proliferation was MS+ 6-BA 2.0 mg·L-1+NAA 0.3 mg·L-1;that the medium for optimal rooting was MS+NAA 0.2 mg·L-1;and a transplanting survival rate up to 95% could be achieved as a result.关键词
水晶花烛/种子处理/无菌播种/组培快繁Key words
Anthurium crystallinum/seed pre-treatment/aseptic sowing/rapid propagation分类
农业科技引用本文复制引用
林辉锋,尚伟,陈昌铭,林发壮,夏朝水,姚凤琴,周辉明..水晶花烛的无菌播种和组培快繁技术研究[J].福建农业学报,2017,32(3):273-277,5.基金项目
福建省星火计划项目(2016S0036) （2016S0036）
