广东医学2017，Vol.38Issue(7)：990-993,4.
硫化氢通过STAT1途径对小鼠酒精性肾间质纤维化的影响
Hydrogen sulfide alleviates alcoholic renal interstitial fibrosis in mice via down-regulating the expression of STAT1
摘要
Abstract
Objective To explore the effects of hydrogen sulfide (H2S) on the alcoholic renal interstitial fibrosis in mice and the expression of signal transducer and activator of transcription 1 (STAT1),metalloproteinase-1 (TIMP-1) and matrix metalloproteinase-24 (MMP-24).Methods Totally 40 male Kunming mice were randomly divided into four groups (n =10),control group,alcoholic group,H2S group,PAG group.Based on standard rat forage in control group,the rats in treatment groups received 4％ ethanol water every day for 12 weeks,while normal water was given in control as placebo.NAHS and PAG treatment were given in H2S group and PAG group,respectively.After 90 days,the pathological changes of kidney tissues were analyzed by PAS staining,and renal interstitial fibrosis were analyzed by Masson staining.The expression of MMP-24,TIMP-1 and STAT1 was analyzed by Western blot.Results Compared with the control group,significant glomerular hypertrophy,tubulointerstitial inflammation and renal interstitial fibrosis were observed in alcoholic group;and the expression levels of MMP-24,TIMP-1 and STAT1 were also significantly increased (P ＜ 0.05).Compared with the alcoholic group,significant alleviation in tubulointerstitial inflammation and renal interstitial fibrosis were revealed H2 S group;with significant down-regulation in MMP-24,TIMP-1 and STAT1 (P ＜ 0.05).Compared with alcoholic group,significantly severer renal interstitial fibrosis was observed in PAG group,with significantly up-regulation of STAT1 and TIMP-1 (P ＜ 0.05).Conclusion H2 S can improve the alcoholic renal interstitial fibrosis in mice,may be related to down-regulating the expression of STAT1 and improve the imbalance of MMP24/TIMP1.关键词
硫化氢/酒精/信号转导和转录激活因子-1/肾间质纤维化/基质金属蛋白酶-24Key words
hydrogen sulfide/alcohol/singnal transducer and activator of transcription 1/renal interstitial fibrosis/matrix metalloproteinase-24引用本文复制引用
刘茂军,李子宁,梁彪,李妍,褚春,杨军..硫化氢通过STAT1途径对小鼠酒精性肾间质纤维化的影响[J].广东医学,2017,38(7):990-993,4.基金项目
国家自然科学基金资助项目(编号:81270181) （编号:81270181）
