广东医学2017，Vol.38Issue(10)：1481-1484,4.
大黄对大鼠脊髓损伤后血-脊髓屏障的保护作用
Protective effect of rhubarb on blood spinal cord barrier after spinal cord injury in rats
摘要
Abstract
Objective To evaluate the effects of Rhubarb on blood spinal cord barrier in rats after spinal cord injury (SCI), looking for a best concentration of Rhubarb decoction lavage and to provide a basis for clinical treatment of SCI.Methods A total of 125 adult healthy SD rats were randomly divided into sham operation group (Sham), model group, and low, medium and high dose groups.Rhubarb solution was given in treatment groups, while normal saline as placebo was given in the other 2 groups.The neurological function of hind limbs was recorded using BBB score.The water content in spinal cord was observed.And using fluorescence microscope to observed the blood spinal cord barrier after injection of Evans blue (EB).Results There were significant differences in BBB scores and water content in spinal cord between control group and model group, rhubarb low, medium and high dose groups (P<0.05).Fluorescence microscope showed that there was no EB stain in control group, while rich EB stain was revealed in model group.The EB stain in rhubarb groups showed reduction in different degreed compared with model group, especially in rhubarb medium dose group.Conclusion In the medium dose group, rhubarb can effectively reduce the permeability of the blood spinal cord barrier in rats after SCI.关键词
脊髓损伤/大黄/血-脊髓屏障Key words
spinal cord injury/rhubarb/decocting concentration/blood spinal cord barrier引用本文复制引用
苑文超,张华,黄桂成,郭杨,袁翰,王磊,周龙云,马勇..大黄对大鼠脊髓损伤后血-脊髓屏障的保护作用[J].广东医学,2017,38(10):1481-1484,4.基金项目
国家自然科学基金面上项目(编号:81573997),江苏省普通高校研究生科研创新计划项目(编号:KYLX16_1147),徐州市2014年推动科技创新后补助备案项目(编号:KC14SH030) （编号:81573997）
