广西植物2017，Vol.37Issue(5)：554-564,11.DOI:10.11931/guihaia.gxzw201608033
一种阳春砂新栽培类型的鉴定
Identification for a new Amomum villosum cultivar
摘要
Abstract
We found a candidate, named Jinqiu, of new Amomum villosum cultivar in October 2010.In order to find out the true identity of Jinqiu, four Amomum germplasms of A.villosum cv.Changguo, A.villosum cv.Yuanguo, A.villosum cv.Zhonghua and A.longiligulare, were planted together with Jinqiu in a field in the University Town of Guangzhou.A series of experiments were conducted to classify Jinqiu subsequently.Morphological traits investigation, RAPD analysis and bioactive compound quantification were carried out among five Amomum germplasms.The results showed that Jinqiu had two types of leaf margins, one was crisped in both sides of leaf blade and the other was half of entire and half of crisped.Changguo, Yuanguo and A.longiligulare had only one type of leaf margin.Changguo and Yuanguo were entire and A.longiligulare was half of entire and half of crisped.The ligule length of Jinqiu was longer than that of Changguo and Yuanguo but shorter than that of A.longiligulare.The date of first flowering of Jinqiu was later than that of Changguo and Yuanguo but earlier than that of A.longiligulare.The fruit shape of Jinqiu was bulbous which was similar to that of Yuanguo and A.longiligulare but different from that of Changguo and Zhonghua, which was long circle.The fruit colour of Jinqiu was dark brown which was different from that of Changguo and Yuanguo but similar to that of Zhonghua and A.longiligulare.The fruit colours of Changguo and Yuanguo were red.The fruit thorn of Junqiu was sharp which was similar to that of Changguo, Yuanguo and Zhonghua, but was different from that of A.longiligulare which was blunt.So Jinqiu and Zhonghua were similar in all biological traits except fruit shape.RAPD-PCR results showed that three primers with abundant polymorphic amplified bands were randomly selected from 91 primers.That of 91.2% polymorphic ratio by one primer indicates there was high possibility of genetic variation in Amomum plants.Genetic similarity coefficients ranged from 0.235 3 to 0.970 6 that means there were differences among the five Amomum germplasms.According to the result of genetic relationship analysis, five germplasms were clustered into two categories: A.longiligulare was in one category and others were in another category.In the latter category, Changguo and Yuanguo were clustered into a small class;Jinqiu and Zhonghua were clustered into another small class.These results revealed that Jinqiu was a new kind of A.villosum cultivar.The weight of per thousand seeds of Jinqiu was the heaviest among that of five germplasms, and the content of volatile oil was also the highest, but the bornyl acetate content in Jinqiu had no significantly difference from others.关键词
锦秋/阳春砂/种质/鉴定/生物学性状/RAPDKey words
Jinqiu/Amomum villosum/germplasm/identification/biological traits/RAPD分类
农业科技引用本文复制引用
蒋烨,苏景,汤丽云,徐杰,陈汝平,邓汝荣,何国振..一种阳春砂新栽培类型的鉴定[J].广西植物,2017,37(5):554-564,11.基金项目
国家科技支撑计划项目(2011BAI01B02) （2011BAI01B02）
海南省产学研一体化项目(CXY20140017) [Supported by the National Key Technology R & D Program of China (2011BAI01B02) （CXY20140017）
Integration of Production, Study and Research Program in Hainan Province (CXY20140017)]. （CXY20140017）
