广西植物2017，Vol.37Issue(5)：587-591,5.DOI:10.11931/guihaia.gxzw201608016
杉木未成熟胚胚性愈伤组织诱导影响因素探析
Exploration and analysis of influence factors of embryogenic callus induction from immature zygotic embryos of Cunninghina lanceolata
摘要
Abstract
Cunninghamia lanceolata is a fast-growing and high-yield timber forest species endemic in China.It has many advantages of fast growth, high production, good quality and wide use, and plays an important role in the forestry production in South China.In this research, an experiment of embryogenic callus induction was carried out.In the experiment, six different genotypes of C.lanceolata improvement species were used as initial explant, and four factors affecting embryogenic callus induction were studied and analysed.These four factors were the key elements on embryogenic callus formation, and they were as follows: genotypes, 6-BA combination, inoculating ways and developmental stages of immature zygotic embryos including.The results showed that the factors of genotypes, 6-BA combination, inoculating ways and developmental stages of immature zygotic embryos all had significant effects on the induction rate of embryogenic callus, but the influence degree each were not identical.Three genotypes in six genotypes obtained embryogenic callus, and one of the genotype (S18) had the highest induction rate of embryogenic callus with 11.7%.When the range of 6-BA combinations was from 1.0 mg·L-1 to 1.5 mg·L-1, the genotype S18 had the highest induction rate.The best method of inoculating was to peel and cut a small hole on end of seed.It was not a good inoculating way to take out zygotic embryos from endosperm, and the zygotic embryos were easy to die in that way.The inoculating method of only cutting a small hole on end of seed and non-peeling was unsuited for embryogenic callus induction.The suitable developmental stage for embryogenic callus induction was from fertilization stage to embryo organ differentiation stage, and it was difficult to obtain embryogenic callus when the zygotic embryos entered into the mature stage.The results indicated that the zygotic embryos which already entered into the mature stage were used as initial explants in the experiment of embryogenic callus induction, and they grew into a complete plant, not callus.关键词
杉木/未成熟胚/胚性愈伤组织/诱导Key words
Cunninghina lanceolata/immature zygotic embryo/embryogenic callus/induction分类
农业科技引用本文复制引用
陈琴,陈代喜,劳广杰,黄开勇,梁机,莫勇德..杉木未成熟胚胚性愈伤组织诱导影响因素探析[J].广西植物,2017,37(5):587-591,5.基金项目
广西优良用材林资源培育重点实验室开放课题(14B0302) （14B0302）
广西自然科学基金(2016GXNSFBA380112) （2016GXNSFBA380112）
广西科学研究与技术开发计划项目(桂科转1599004-9)[Supported by Guangxi Key Laboratory Open Program of Superior Timber Trees Resource Cultivation(14B0302), Guangxi Natural Science Foundation(2016GXNSFBA380112) （桂科转1599004-9）
Guangxi Science Research and Technology Development Program (1599004-9)]. （1599004-9）
