广西植物2017，Vol.37Issue(5)：592-598,7.DOI:10.11931/guihaia.gxzw201604016
不同光源对柚木组培苗生长发育的影响
Effects of various LED lights on growth and development of Tectona grandis plantlets in vitro
摘要
Abstract
The development and application of light-emitting-diodes(LEDs) provide more light sources for plant tissue culture.In this study, the subculture plantlets of Tectona grandis were treated by different LEDs, then the growth index(plant height, leaf width, fresh leaf mass and whole-plant dry weight) and chlorophyll content were measured and the leaf surface structures were observed underelectron microscopy, in order to discover the effects of LED lights on the growth and development of plantlets and to find the optimal light environment for their rapid growth.The results showed that the plantlet height was HFL﹥H1RB﹥HS﹥H2RB﹥H3RB﹥HD and the axillary bud height was hFL﹥hs﹥hd﹥h1RB﹥h2RB﹥h3RB, under the following six different lights: fluorescent lamp(FL), three mixtures of R plus B LED(1RB, 2RB, 3RB), full spectrum light LED(S) and scattered light(D).The maximum leaf width was significantly larger in plantlets that were cultured under 2RB and 3RB than those under FL, S and D.Moreover, the maximum leaf width and fresh leaf mass increased significantly aslight intensity of RB increased.Undercondition D and S lights, the plant dry weights were significantly lighter than those under the other LEDs.Chlorophyll contents were greater in plantlets under RB and S than those under condition FL and condition D.The adaxial epidermal cells of plantlets under 2RB and 3RB were in the shape of irregular polygonand inlaid each other, while those under other conditions appeared in the shape of circles.The stomata amounts on the abaxial of plantlets under lights(FL, RB, S) were 1.5 times more than that under no lighting equipment(D).The stomatal pores of plantlets under 2RB and 3RB opened more widely than those under condition FL and D, the guard cells appeared as well-type protuberance.Overall, the fluorescent light and the full spectrum light LED were suitable for Tectona grandis multiplication culture according to significant height growth of plantlets and fast differentiation of axillary bud, and further contributed to increase the proliferation rate.On the other hand, mixtures of R plus B LED (3RB, 2RB) were beneficial in strong seedling culture with big leaf sizes, strong seedling, high biomass, fully-developed leaf epidermal structure, contributing to increase photosynthesis.关键词
柚木组培苗/光照/生长/叶表面结构Key words
Tectona grandis plantlets/illumination/growth/leaf surface structure分类
农业科技引用本文复制引用
裘珍飞,曾炳山,郭光生,刘英..不同光源对柚木组培苗生长发育的影响[J].广西植物,2017,37(5):592-598,7.基金项目
国家"十二五"科技支撑计划项目(2012BAD21B01)[Supported by National Science and Technology Support Program of the 12th Five-year Plan(2012BAD21B01)]. （2012BAD21B01）
