工业微生物2017，Vol.47Issue(2)：1-6,6.DOI:10.3969/j.issn.1001-6678.2017.02.001
氯化锂诱变选育3-羟基丙酸高产菌株
Breeding of 3-hydroxypropnic acid high-producing strain by lithium chloride mutagenesis
摘要
Abstract
In this study,Debaryomyces hansenii DH01 was used as original strain and LiCl was chosen to generate mutations.The mutant strains of 3-hydroxypropinic acid (3HP) high-producing strain were screened out for the practical production.After treatment with LiCl twice,a mutant strain designated as wt06 was obtained.When fermented at 28 ℃,120 r/min for 48 h,wt06 could produce 23.70 g/L of 3HP,which was 5.44 times as high as that of the original strain DH01.Furthermore,the mutant strain wt06 had good heredity stability.关键词
3-羟基丙酸(3HP)/LiCl诱变/翰逊德巴利酵母菌/遗传稳定性Key words
3-hydroxypropinic acid (3HP)/lithium chloride mutagenesis/Debaryomyces hansenii/heredity stability引用本文复制引用
王陶,谢平进,董玉玮,高明侠,张传丽,李文,夏霞,吕彬..氯化锂诱变选育3-羟基丙酸高产菌株[J].工业微生物,2017,47(2):1-6,6.基金项目
江苏省产学研联合创新资金-前瞻性联合研究项目(BY2014031)、徐州市科技计划项目(KC14SM094)、徐州工程学院重点培育课题(XKY2015104)和江苏省“青蓝工程”、“333”工程科研项目(BRA2016274)资助. （BY2014031）
