工业微生物2017，Vol.47Issue(2)：11-17,7.DOI:10.3969/j.issn.1001-6678.2017.02.003
枯草芽孢杆菌HS-A38产抗菌脂肽Bacillomycin D的组分分析及鉴定
Analysis and identification of lipopeptide Bacillomycin D produced by Bacillus subtilis HS-A38
摘要
Abstract
In this study the lipopeptide compounds produced by the strain Bacillus subtilis HS-A38 was isolated and identified,and their antibacterial activities were analyzed.The lipopeptide crude extract LP was isolated by using the acid precipitation and organic solvent extraction from the strain HS-A38 fermentation culture,and its yield was 1.956 g/L.Four components were identified by the ninhydrin color printing and thin layer chromatography,named as LP1,LP2,LP3 and LP4.The results of antibacterial activity test indicated that LP3 had higher activity against two marine pathogenic bacteria of Vibrio parahaemolyticus and Pseudomonas aeruginosa.The LP3 was separated by the Silica Gel Column Chromatography,and then further purified and identified by using RP-HPLC and MALDI-TOF-MS.According to the RPHPLC analysis,the peak group of LP3-1 was found within 20 min ～ 28 min.Based on the mass spectra analysis,the main composition of the LP3-1 was Bacillomycin D.关键词
枯草芽孢杆菌/脂肽类物质/分离纯化/抑菌活性Key words
Bacillus subtilis/lipopeptide/isolation and purification/antibacterial activity引用本文复制引用
刘佳欣,丛丽娜,李成,董亮,张永吉,齐建权..枯草芽孢杆菌HS-A38产抗菌脂肽Bacillomycin D的组分分析及鉴定[J].工业微生物,2017,47(2):11-17,7.基金项目
辽宁省自然科学基金项目(201602047) （201602047）
国家海洋公益性行业科研专项项目(201405003) （201405003）
辽宁省教育厅重点实验室(LZ2014029). （LZ2014029）
