工业微生物2017，Vol.47Issue(2)：18-23,6.DOI:10.3969/j.issn.1001-6678.2017.02.004
不同培养方式对牛樟芝活性组分的影响
Influences of cultivation methods on active components of Antrodia camphorata
摘要
Abstract
More attention was paid to Antrodia camphorata due to its anti-cancer and hepatoprotective effects.In this study,the influences of solid-state fermentation and different submerged fermentation methods on the active components of A.camphorata were investigated.The results showed that fermented grain types had obvious effects on the synthesis of active products.HighLand barley was the best substrate with high various active components.As a result,the yields of antrodin C and antroquinonol were as high as 5 901.27 mg/kg and 3 715.76 mg/kg,respectively.In conventional submerged fermentation,the antrodins compounds,mainly antrodin C,were in the mycelia of A.camphorata.However,antroquinonol compounds could not be produced.By adding its precursor of coenzyme Q0,antroquinonol could be biosynthesized in the submerged fermentation.Therefore,the vegetable oil was added during the synchronous extractive fermentation,significantly improving the active component types and yield.Besides antroquinonol,great amount of antroquinonol B was synthesized.Most active ingredients were enriched and extracted continually,among them the antrodin C and antroquinonol production reached 289.80 mg/L and 95.39 mg/L,respectively.The antrodin C production was increased by 7.06 times by comparing to that of the conventional submerged fermentation,while the antroquinonol production was increased by 203.89％ by comparing to that of the inducing fermentation.关键词
牛樟芝/固态发酵/液态发酵/Antrodin C/AntroquinonolKey words
Antrodia camphorata/solid-state fermentation/submerged fermentation/antrodin C/antroquinonol引用本文复制引用
周璇,夏永军,刘胜男,崔涵婧,初宁,胡怡欣,肖睿,张钰妍,艾连中..不同培养方式对牛樟芝活性组分的影响[J].工业微生物,2017,47(2):18-23,6.基金项目
上海市自然科学基金项目(15ZR1428900) （15ZR1428900）
上海市大学生创新创业训练计划项目(SH2016128) （SH2016128）
沪江基金研究基地专项(D15012). （D15012）
