工业微生物2017，Vol.47Issue(2)：24-28,5.DOI:10.3969/j.issn.1001-6678.2017.02.005
腺苷高产菌株Bacillus subtilis DI4-24退化的遗传机制与控制研究
Degeneration and control of adenosine high-yielding strains of Bacillus subtilis DI4-24
任韶霞 1刘磊 1常利草 2李金鑫 1刘国生1
作者信息
- 1. 河南师范大学生命科学学院,河南新乡453007
- 2. 功能微生物绿色转化技术河南省工程实验室
- 折叠
摘要
Abstract
The high adenosine-producing strain Bacillus subtilis DI4-24 as an artificial mutant degenerated easily during preservation,consecutive subculture and fermentation.The genetic traits of reverse mutant were analyzed to study the degeneration mechanism,and the effects of physical and chemical factors on reverse mutation were investigated to find the control method.The mutants were screened on selective medium and its genotype was analyzed by growth spectrum method.The red materials accumulated in mutation ceils were measured by spectrophotometry in order to analyze the influence factors of reverse mutation.Histidine typhimurium reverse nutation caused degeneration adenosine production declined dramatically for the reverse mutation strain.It could effectively slow down the recession by preserving culture at low temperature and reducing generations of propagation.During the process of seed preparation,reducing culture temperature by 2 ℃ ～3 ℃,shortening the culture time to 8 h,adjusting medium pH to mildly acidic (6.5),and adding substances such as carotene and folic acid in the seed culture medium could significantly reduce the reverse mutation rate.Under above conditions,the seed production performance was improved,and the adenosine production was increased by 9％ if compared with that of the control.关键词
腺苷/菌种退化/组氨酸缺陷型/回复突变Key words
adenosine/degeneration/histidine gene deficiency/reverse mutation引用本文复制引用
任韶霞,刘磊,常利草,李金鑫,刘国生..腺苷高产菌株Bacillus subtilis DI4-24退化的遗传机制与控制研究[J].工业微生物,2017,47(2):24-28,5.
