工业微生物2017，Vol.47Issue(2)：29-36,8.DOI:10.3969/j.issn.1001-6678.2017.02.006
聚乙烯醇-明胶复合膜固定化重组大肠杆菌NAD激酶的研究
Immobilization of recombinant NAD kinase in E.coli by polyvinyl alcohol-gelatin composite membrane
袁飞飞 1李红梅 1侯堃1
作者信息
- 1. 上海理工大学医疗器械与食品学院,上海 200093
- 折叠
摘要
Abstract
The NAD kinase was immobilized on the composite membrane to improve its stability.Polyvinyl alcohol (PVA),polylactic acid (PLA),sodium alginate (SA) and gelatin (GEL) membrane were used as immobilized carrier.Based on the single-factor tests,the optinal immobilization conditions were determined as follows:PVA∶ GEL =4∶ 1,enzyme concentration of 0.6 mL,immobilization time of 6 h,immobilization temperature of 35 ℃.Under the optimal imnobilization conditions,the activity recovery was up to 84％.Properties of the immobilized NAD kinase were investigated and were compared with those of the free enzyme.After immobilization,the optimum reaction temperature was increased from 50℃ to 55℃,the optimum reaction pH was decreased from 8.0 to 7.0,the pH stability and thermal stability of immobilized enzyme were superior to those of free enzyme,but the affinity of the immobilized enzyme was decreased.After the immobilized enzyme was reused for 6 times,the enzyme activity could maintaiu more than 75％ of the initial enzyme activity.The results showed that the PVA-GEL composite membrane immobilized enzyme had good operation stability.关键词
聚乙烯醇-明胶/NAD激酶/固定化Key words
PVA-GEL/NAD kinase/immobilization引用本文复制引用
袁飞飞,李红梅,侯堃..聚乙烯醇-明胶复合膜固定化重组大肠杆菌NAD激酶的研究[J].工业微生物,2017,47(2):29-36,8.
