郑州大学学报（医学版）2017，Vol.52Issue(3)：247-250,4.DOI:10.13705/j.issn.1671-6825.2017.03.002
食管鳞癌细胞系TE7放射照射后miRNAs及DNA修复相关因子的表达
Expressions of miRNAs and DNA damage repair factors in esophageal squamous cell carcinoma cell line TE7 after X-ray radiation
摘要
Abstract
Aim: To explore the expressions of miRNAs and DNA repair factors in esophageal squamous cell carcinoma cell line TE7 after radiation.Methods: Real-time PCR was used to detect the expressions of miR-126, miR-21, miR-101, DNA-PKcs, and RAD21 mRNA in TE7 cells treated with different doses(0, 2, 4, 6, 8 Gy) of X-ray radiation, and Western blot was used to detect the expressions of DNA-PKcs and RAD21 protein.Results: In TE7 cells treated with X-ray radiation, the expressions of miR-126, DNA-PKcs and RAD21 mRNA were up-regulated with the dose increasing(P<0.05), that of miR-101 was down-regulated (P<0.05), and the expressions of phospho-DNA-PKcs, total DNA-PKcs and RAD21 protein had no statistical changes(P>0.05).The expressions of miR-126 and miR-101 were negatively correlated with that of RAD21 protein(r=-0.899,-0.853,P<0.05) in TE7 cells treated with 4 Gy radiation.Conclusion: MiR-126 and miR-101 may influence the radio-sensitivity of TE7 cells by regulating the expression of RAD21.关键词
食管癌/miR-126/miR-21/miR-101/DNA-PKcs/RAD21/放射照射Key words
esophageal carcinoma/miR-126/miR-21/miR-101/DNA-PKcs/RAD21/radiation分类
医药卫生引用本文复制引用
赵晓静,马军,袁翎,王昆仑,李南,张中冕,孙佳..食管鳞癌细胞系TE7放射照射后miRNAs及DNA修复相关因子的表达[J].郑州大学学报（医学版）,2017,52(3):247-250,4.基金项目
河南省科技厅科技攻关项目 152300410164 （）
