郑州大学学报（医学版）2017，Vol.52Issue(3)：251-254,4.DOI:10.13705/j.issn.1671-6825.2017.03.003
α-细辛醚诱导的Eca-109细胞中cytC、bax的表达
Expressions of cytochrome C and bax on Eca-109 cells inducing by α-asarone
摘要
Abstract
Aim: To observe the influence of α-asarone on esophageal carcinoma Eca-109 cells apoptosis and cytochrome c(cytC), bax expression, and explore its molecular mechanism.Methods: 5-FU and different dosages of α-asarone were used to intervene culture Eca-109 cells in vitro.morphologic changes were viewed under an inverted fluorescence microscope after DAPI staining assay.Cell proliferation and apoptosis rate were measured by MTT and Annexin V-PI.The protein and mRNA expression levels of cytC and bax were detected by Western blot and real-time quantitative PCR.Results: Compared with blank group,cell proliferation rate decreased significantly, and cell apoptosis rate increased significantly in 5-FU and different dosages of α-asarone groups(P<0.05), the expression levels of cytC and bax protein and mRNA increased significantly in 5-FU different dosages of α-asarone groups(P<0.05).Conclusion: α-asarone could inhibit Eca-109 cells proliferation and induce cell apoptosis via up-regulating cytC and bax′s expression.关键词
α-细辛醚/细胞色素C/bax/Eca-109细胞Key words
α-asarone/cytochrome C/bax/Eca-109 cell分类
医药卫生引用本文复制引用
张妍,韩倩倩,朱艳琴..α-细辛醚诱导的Eca-109细胞中cytC、bax的表达[J].郑州大学学报（医学版）,2017,52(3):251-254,4.基金项目
河南省基础与前沿技术研究项目 072300450030 （）
河南省科学技术研究重点项目 13A310612 （）
河南省教育厅高等学校重点科研项目 16A310021 （）
