郑州大学学报（医学版）2017，Vol.52Issue(3)：259-263,5.DOI:10.13705/j.issn.1671-6825.2017.03.005
慢病毒介导的FOXQ1过表达对人脐带间充质干细胞衰老的影响
Effects of lentivirus-mediated FOXQ1 overexpression on senescence of hUC-MSCs
摘要
Abstract
Aim: To construct the FOXQ1 overexpression lentivirus vector and explore the effect of FOXQ1 overexpression on human umbilical cord mesenchymal stem cells(hUC-MSCs) senescence.Methods: After amplification of FOXQ1 sequences, the recombinant lentivirus vector(pHBLV-FOXQ1-Puro) was constructed.The hUC-MSCs overexpressing FOXQ1 infected by the prepared vector and screened by puromycin screening.The hUC-MSCs infected by the empty vector were used as control.The protein and mRNA expression levels of FOXQ1 were measured by Western blot and qPCR.CCK-8 assay was used to test the ability of cell proliferation.SA-β-Gal and AP staining assay were used to detect the effect of FOXQ1 overexpression on cell senescence.Results: pHBLV-FOXQ1-Puro recombinant vector was constructed successfully, and its infection was effective.The protein and mRNA expression levels of FOXQ1 overexpression group were significantly higher than control group (P<0.05).CCK-8 results showed that FOXQ1 overexpression could enhance the ability of cell proliferation.SA-β-Gal and AP staining results showed that FOXQ1 overexpression could delay senescence of hUC-MSCs.Conclusion: The hUC-MSCs overexpressing FOXQ1 have been successfully constructed, and FOXQ1 overexpression could delay the senescence of hUC-MSCs.关键词
FOXQ1/过表达/慢病毒载体/人脐带间充质干细胞/衰老Key words
FOXQ1/overexpression/lentivirus vector/hUC-MSC/senescence分类
生物科学引用本文复制引用
张涛,王攀,马珊珊,石振庆,程康,黄团结,刘艳霞,杨波,关方霞..慢病毒介导的FOXQ1过表达对人脐带间充质干细胞衰老的影响[J].郑州大学学报（医学版）,2017,52(3):259-263,5.基金项目
国家自然科学基金资助项目 81471306,81501200 （）
河南省高校科技创新团队基金资助项目 15IRTSTHN022 （）
河南省科技创新人才计划项目 154200510008 （）
中国博士后科学基金面上资助项目 2015M572122 （）
国家教育部高等学校博士学科点专项科研基金项目 20114101110004 （）
