郑州大学学报（医学版）2017，Vol.52Issue(3)：263-267,5.DOI:10.13705/j.issn.1671-6825.2017.03.006
余甘根苷B对柯萨奇病毒B3感染的心肌细胞及Balb/c小鼠的保护作用
Protection effect of phyllaemblicin B on coxsackie virus B3 infected myocardiocytes and Balb/c mice
摘要
Abstract
Aim: To investigate the protection effect of phyllaemblicin B on myocardiocytes and Balb/c mice infected with coxsackie virus B3(CVB3).Methods: Viral myocarditis(VMC) models infected with CVB3 were established in myocardiocytes and Balb/c mice.Cell survival rate was measured by cytopathic effect(CPE) assay, the contents of aspartate transferase(AST) and lactate dehydrogenase(LDH) were determined in supernatant of myocardiocytes culture medium.Histopathological changes of myocardium of Balb/c mice infected with CVB3 were analyzed to test the antiviral activity of phyllaemblicin B.Results: CVB3 infection induced obvious CPE in myocardiocytes in vitro.After treatment with phyllaemblicin B, CPE on myocardiocytes infected with CVB3 was obviously inhibited, meanwhile the activities of AST and LDH were dramatically reduced compare with the untreatment group(P<0.05).Ultra-structure of mouse myocardium demonstrated that CVB3 infection led to pathological lesions including cardiac muscle fibers breakdown and destruction of nucleus structure.With the treatment of phyllaemblicin B, the histopathology changes in mice myocardium were alleviated.Conclusion: Phyllaemblicin B has obvious protection effect on myocardiocytes and Balb/c mice infected with CVB3.关键词
余甘根苷B/柯萨奇病毒B3/心肌细胞Key words
phyllaemblicin B/coxsackie virus B3/myocardiocyte分类
医药卫生引用本文复制引用
王鹏举,彭有梅,张艳,江金花,范程程,娄晓月,陈文娟,王亚峰..余甘根苷B对柯萨奇病毒B3感染的心肌细胞及Balb/c小鼠的保护作用[J].郑州大学学报（医学版）,2017,52(3):263-267,5.基金项目
国家自然科学基金青年科学基金项目 81302813 （）
