郑州大学学报（医学版）2017，Vol.52Issue(3)：271-274,4.DOI:10.13705/j.issn.1671-6825.2017.03.008
上调FOXC1的表达对SKOV3细胞增殖及侵袭的影响
Impact of up-regulating FOXC1 expression on proliferation and invasion of SKOV3 cells
摘要
Abstract
Aim: To explore the changes of proliferation and invasion in SKOV3 cells after up-regulating the expression of FOXC1.Methods: SKOV3 cells were divided into 3 groups: cells in experimental group were infected with p-EGFP-lentiviral vector with overexpressed FOXC1 plasmids, cells in vector control group were infected with empty p-EGFP-lentiviral vector, and cells in blank control group, not infected.The expressions of FOXC1 mRNA and protein were detected by qRT-PCR and Western blot,respectively.The cell proliferation after 24,48 and 72 h infection and cell invasion after 24 h infection were observed by CCK-8 kit and Transwell invasion assay,respectively.Results: The expressions of FOXC1 mRNA and protein in SKOV3 cells in experimental group were significantly higher than those in the vector control and blank control groups(P<0.05).Compared with blank control group and vector control group, the proliferation activity of SKOV3 cells in experimental group was reduced at different time points, so was the invasive ability(P<0.05).Conclusion: Up-regulation of FOXC1 may suppress cell proliferation and invasion of SKOV3 cells.关键词
SKOV3细胞/FOXC1/增殖/侵袭Key words
SKOV3 cell/FOXC1/proliferation/invasion分类
医药卫生引用本文复制引用
任琛琛,刘泇希,杨立,薛景戈,李飞燕,刘灵,李静,白杨..上调FOXC1的表达对SKOV3细胞增殖及侵袭的影响[J].郑州大学学报（医学版）,2017,52(3):271-274,4.基金项目
河南省科技厅科技攻关项目 162102310131 （）
