黑龙江农业科学Issue(4)：9-11,3.DOI:10.11942/j.issn1002-2767.2017.04.0009
蝴蝶兰高效再生体系与遗传转化体系的建立
Establishment of Efficient Regeneration System and Genetic Transformation System of Phalaenopsis
盛慧1
作者信息
- 1. 哈尔滨市农业科学院 生物中心,黑龙江 哈尔滨 150029
- 折叠
摘要
Abstract
In order to establish the regeneration system and genetic transformation system of Phalaenopsis amabilis,Phalaenopsis amabilis was used for artificial pollination,after maturation,the embryos were seeded on the induction medium to induce protocorm or adventitious buds.The type and concentration of exogenous regulators were explored,and the concentration of kanamycin and the type and concentration of the fungicide were screened.The results showed that when the concentration of 6-BA was 5 mg·L.-1 and NAA was 1 mg·L.-1,the induction efficiency of protocorm was the highest.When the concentration of 6-BA 1.5 mg·L.-1,while adding 0.5 g·L.-1 activated carbon and 40 mL·L.-1 coconut,the best effect of strong seedlings.The results showed that the concentration of kanamycin was 4 mg·L.-1,the type of fungicide was amoxicillin clavulanate (7:1),and the concentration was 100 mg·L.-1.关键词
蝴蝶兰/再生体系/遗传转化Key words
Phalaenopsis/regeneration system/genetic transformation分类
农业科技引用本文复制引用
盛慧..蝴蝶兰高效再生体系与遗传转化体系的建立[J].黑龙江农业科学,2017,(4):9-11,3.
