中国实用神经疾病杂志2017，Vol.20Issue(11)：1-4,4.
新型Rho激酶抑制剂FSD-C10对A53T细胞突起的作用
The effect of novel Rho kinase inhibitor FSD-C10 on neurite of A53T cell
摘要
Abstract
Objective To investigate the effect of novel Rho kinase inhibitor FSD-C10 on neurite of A53T cell and its possible mechanism of the neurite outgrowth.Methods A53T cells were routinely cultured and divided into three groups:PBS group,Fasudil group and FSD-C10 group.The neurite length of A53T cells was measured by Image-Pro Plus v6.0 at different time points.The expressions of synapsinⅠ,synaptophysin and PSD-95 protein were examined by Western blot.Results Fasudil and PSD-C10 promoted neurite outgrowth of A53T cells.The expression of synapsinⅠ protein in A53T cells was increased,especially in FSD-C10-treated A53T cells.Both Fasudil and FSD-C10 slightly stimulated the PSD-95 at early state,but inhibited it at late state.Conclusion FSD-C10 can promote neurite outgrowth of A53Tcells possibly by its effect on the expression of synapsinⅠ protein.关键词
FSD-C10/A53T细胞/突起/synapsinⅠKey words
FSD-C10/A53T cells/Neurite/SynapsinⅠ分类
医药卫生引用本文复制引用
赵静,张伟,马杰,张海飞,尉杰忠,柴智,宋丽娟,肖保国,马存根..新型Rho激酶抑制剂FSD-C10对A53T细胞突起的作用[J].中国实用神经疾病杂志,2017,20(11):1-4,4.基金项目
国家自然科学基金2012年面上项目(81272163) （81272163）
山西省回国留学人员重点科研资助项目(2014-重点7) （2014-重点7）
山西中医学院"2011"培育计划项目(2011PY-1) （2011PY-1）
