家畜生态学报2017，Vol.38Issue(4)：48-52,67,6.
高寒草甸牧草与牦牛瘤胃液氨基酸构成及相关性分析
Composition of Amino Acids in Rumen Fluid of Yak and its Correlation with Forage in Alpine Medaow
摘要
Abstract
As the alpine meadow pasture being the study subject,the experiment analyzed the protein and amino acid (AA) content and the AA composition in rumen fluid,as well as the correlation among them,aiming to provide technical support for the scientific management of alpine grassland system.Three health grazing lactating yaks were taken to collect the rumen fluid;meanwhile the grass samples were collected to determine the protein and amino acid contents.The results showed that:(1) Crude protein content (CP),soluble crude protein (SCP),and non-protein nitrogen (NPN) was 147.54 g/kg,49.28 g/kg,and 1.37 g/kg in native grass,respectively.(2) Total amino acids (TAA) in grass and rumen fluid were 1186.26 mg/kg and 53.70 mg/L.The ratios of essential AA to TAA (E/T) and EAA to non-essential AA (E/N) were 37.7％ and 60.6％ in native grass,respectively.(3) In native grass,Cystine and CP content were negatively correlated,so were Glutamic acid and SCP (P＜0.05),but Serine and NPN content were positively correlated (P＜0.05),and there was no significant correlation between the other AA (P＞0.05).(4) The concentration of CP and SCP in grass,respectively,were of positive correlation with Phenylalanine and Aspartic acid,and Glycine,Alanine,Lysine,Histidine,Cystine and Tryptophan in the rumen fluid (P＜0.05);(5) The concentration of AA in grass and yak rumen fluid were of positive correlation (P＜0.01;Methionine,P＜0.05).In summary,yaks can obtain the necessary nutrients from summer pasture,and the AA composition for grazing yak's rumen fluid were affected by protein content in native grass.And therefore,further researches should be aimed at amino acid metabolism,and characteristics of AA output from yak wool,milk and other products to explore the mechanism of grazing yaks AA nutrition.关键词
牦牛/可食性牧草/瘤胃液/氨基酸/相关性Key words
Yak/edible forage/rumen fluid/amino acids/correlation分类
农业科技引用本文复制引用
付晓悦,张霞,王虎成..高寒草甸牧草与牦牛瘤胃液氨基酸构成及相关性分析[J].家畜生态学报,2017,38(4):48-52,67,6.基金项目
国家自然基金项目(31302001) （31302001）
中央高校基本科研业务费(lzujbky-2016-1) （lzujbky-2016-1）
