江西农业大学学报2017，Vol.39Issue(2)：402-408,7.DOI:10.13836/j.jjau.2017052
濒危桃儿七根中产鬼臼毒素内生真菌的筛选及分子鉴定
Screening and molecular identification of podophyllotof xin-producing endophytic ungi from root of endangered Sinopodophyllumhexandrum
摘要
Abstract
The root and rhizome of endangered Tibetan Sinopodophyllumhexandrum contains podophyllotoxin,screening and identification of endophytic fungi from rootof Sinopodophyllumhexandrum in were carried out to obtain endophytic strains producing podophyllotoxin compounds and provide the reference to alleviate the shortage of the medicinal resources and protect plant resources and discover new natural activitystrains.The endophytic fungi from root of Sinopodophyllumhexandrum were isolated by using the tissue method,the metabolites of podophyllotoxin compounds were detected by thin layer chromatography and high performance liquid chromatography,the detection wave length was 254 nm,mobile phase was acetonitrile-water (40∶60).Combined with morphological and molecular biology methods the podophyllotoxin compounds, which produced endophytic fungi were identified.8 strains of endophytic fungi were isolated,in which G-1,G-2,G-3,G-4,G-5 strains in the fermentation broth contained podophyllotoxin compounds.They were identified as Pleosporales sp.MF565 (100%),Pseudallescheria sp.T55 (100%),Hypocreales sp.MF489 (100%),Hypocreales sp.LF213 (100%) and Cyphellophorareptans strain CBS 458.92 (100%).关键词
桃儿七/内生真菌/鬼臼毒素/分子鉴定Key words
Sinopodophyllumhexandrum/endophytic fungi/podophyllotoxin/molecular identification分类
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王婷,马越兴,叶耀辉,郑红梅,张博文,张恩慧..濒危桃儿七根中产鬼臼毒素内生真菌的筛选及分子鉴定[J].江西农业大学学报,2017,39(2):402-408,7.基金项目
江西省自然科学基金项目(20132BAB205080)和江西省专利研发引导与产业化示范专项(省自然科学基金计划)(20143BBM26057) Project supported by Natural Fund Project of Jiangxi Province(20132BAB205080) and Patent Development Guide and Industrialization Demonstration Projects in Jiangxi Province(Province Natural Science Foundation Project)(20143BBM26057) （20132BAB205080）
