昆明医科大学学报2017，Vol.38Issue(1)：8-12,5.
呼吸道病原体核酸恒温扩增芯片十三联检在下呼吸道感染常见病原体基因中的检测
The Application of Loop-mediated Amplification Chip in Gene Testing of Common Lower Respiratory Pathogens
摘要
Abstract
Objective To investigate the value of Loop-mediated Amplification (LAMP) in the gene diagnosis of common pathogens in lower respiratory infection.Methods One hundred and twenty-two cases of lower respiratory tract secretions and bronchoalveolar lavage fluid were collected from patients with suspected lower respiratory infection.The pathogens were tested by using LAMP,isolation and cultivation of microbial pathogens,real-time FQ-PCR.The results were compared with different specimen types,age and detection methods.Statistical analysis was performed using the SPSS software and Kappa test.Results In 122 cases of specimens from patients with respiratory infection,the positive rate of sputum was higher (55.56％) than that in bronchoalveolar lavage fluid (46.88％).The main pathogens were Methicillin-resistant Staphylococcus (MASA) and mycoplasma pneumoniae (MP) (P＜0.05) respectively.In different age stage,MASA was mainly detected in the elder group (＞60 years),and MP was the main pathogen in the younger patients (≤20 years) (P＜0.01).The detection rate of LAMP (50.00％) was higher than the microbial Isolation and cultivation (36.67％).The detection rates of Klebsiella pneumoniae (Kpn) and Bauman Acinetobacter (CSAB) by two different methods were consistent (kappa＞0.4),however the consistencies in other pathogens were low (kappa＜0.4).And there was a high consistency between LAMP and real-time FQ-PCR in the detection of MP (kappa ＞0.7).Conclusions LAMP has the advantages of rapidity,sensitivity,high effciency and specificity,high throughput and wide coverage of pathogens,which may be a quick and reliable laboratory diagnosis method.And using different respiratory specimens or methods may have different results.They have their own advantages and disadvantages,which are needed to be paid close attention by clinicians.关键词
下呼吸道感染/病原体/环介导恒温扩增Key words
Lower respiratory infection/Pathogen/Loop-mediated amplification分类
医药卫生引用本文复制引用
唐睿珠,罗正琼,徐秋月,赵滢,李欢,王云娟,宋宇,刘子杰..呼吸道病原体核酸恒温扩增芯片十三联检在下呼吸道感染常见病原体基因中的检测[J].昆明医科大学学报,2017,38(1):8-12,5.基金项目
国家自然科学基金资助项目(81460357) （81460357）
