昆明医科大学学报2017，Vol.38Issue(2)：33-37,5.
干扰Nrf2提高二氢青蒿素对结肠癌细胞增殖的抑制作用
Interfering RNA-mediated Nrf2 Gene Knockdown Enhances DHA Induced Cytotoxity in Colon Cancer Cells
摘要
Abstract
Objective To investigate the effect of Nrf2 gene knockdown on DHA-induced cytotoxity in Compared with Con group and shNon group,there was significant difference human colon carcinoma HCT-116 cells.Methods The inhibition changes in the cell viability after treatment with different concentration of DHA were detected by MTT assay and the ROS was assayed by flow cytometry.AnnexinV-FITC apoptosis kit was used to detect the cell apoptosis.Nrf2 protein expression transfected with the shRNA targeting Nrf2 was analyzed with western blotting.Results The inhibitory rate of shNRF2 group was 24.05％ when DHA concentration was 5 μ mol / L,and 31.32％ when DHA concentration reached 10 μmol/L.When DHA rose to 20 μ mol/L,the inhibition rate climbed to 46.18％,compared with Con group and shNon group,there was significant difference.The ROS level in HCT-116 cells was found to increase by interfering with Nrf2.NAC reversed Nrf2 interference and DHA-induced ROS increased.It was detected by flow cytometry that the apoptosis rate of HCT-116 cells was induced by inhibiting Nrf2.Conclusion DHA can increase the proliferation of HCT-116 cells by increasing the ROS in cells,and can also increase the ROS content and enhance the apoptosis-inducing effect of DHA after knockdowning Nrf2.关键词
二氢青蒿素/Nrf2/活性氧/凋亡Key words
Dihydroartemisinin/Nrf2/Reactive oxygen species/Apoptosis分类
医药卫生引用本文复制引用
纳鑫,陈丽君,罗敏,陈亚娟,卿晨..干扰Nrf2提高二氢青蒿素对结肠癌细胞增殖的抑制作用[J].昆明医科大学学报,2017,38(2):33-37,5.基金项目
云南省应用基础研究计划项目(2013FZ061) （2013FZ061）
