临床口腔医学杂志2017，Vol.33Issue(5)：259-262,4.DOI:10.3969/j.issn.1003-1634.2017.05.002
洗必泰溶液对不同时期粪肠球菌生物膜作用效果的实验研究
Effects of chlorhexidine solution on different stages of Enterococcus faecalis biofilms
摘要
Abstract
Objective:To evaluate the antibacterial effect of chlorhexidine(CHX)solution as being one of the most common root canal irrigation medication on different stages of Enterococcus faecalis biofilms.Methods:Enterococcus faecalis biofilms were grown upon the hydroxyapatite disks which were coated with bovine dermal type I collagen in brain-heart infusion broth respectively for 24 hours and 3 weeks.Then biofilms of different stage were subjected to 5 minutes exposure to 2% chlorhexidine or normal saline(NS).After treatment,the volume ratio of dead bacteria in biofilms was assessed by confocal laser scanning microscopy(CLSM)by using a LIVE/DEAD viability stain.Biofilm structure was visualized by scanning electron microscopy(SEM).Results:When appling 2% CHX solution,on the one hand bacterial cells in biofilms of 24 hours were destructed obviously.On the other hand,extracellular polymeric substances(EPS)in 3 weeks biofilms were obliterated.The proportion of killed bacteria in young biofilms(24 hous)and mature biofilms(3 weeks)was respectively(38.6±2.0)% and(86.0±3.0)%,while normal saline did not have the ability to kill bacteria(P<0.05).Conclusion:2% CHX solution has a outstanding antibacterial effect against different stages of Enterococcus faecalis biofilms,presenting a cell body destruction function in young biofilms and a obliteration function of extracellular polymeric substances in mature biofilms.关键词
粪肠球菌生物膜/洗必泰溶液/根管消毒/胞外聚合物Key words
Enterococcus faecalis biofilms/Chlorhexidine solution/Root canal disinfection/Extracellular polymeric substances分类
医药卫生引用本文复制引用
唐学智,杜田丰,石琦,丁晓晓,姚怡辰,曹颖光..洗必泰溶液对不同时期粪肠球菌生物膜作用效果的实验研究[J].临床口腔医学杂志,2017,33(5):259-262,4.基金项目
国家自然科学基金项目(81500847) （81500847）
