林业科学2017，Vol.53Issue(4)：56-64,9.DOI:10.11707/j.1001-7488.20170407
土壤残存草甘膦对杉木幼苗生理及养分吸收的影响
Effect of Glyphosate Residues in Soil on Physiological Characteristics and Nutrient Absorption of Chinese fir Seedlings
摘要
Abstract
[Objective]Glyphosate is usually more or less left over forest land after using for weeding.To explore effects of the glyphosate residues on Chinese fir, a pot experiment was conducted to determine the physiological mechanism of Chinese fir in responses to residual glyphosate in soil, and the effect of glyphosate on the nutrient uptake of Chinese fir.This study aimed to provide the theoretical basis for the rational application of glyphosate in forestry production, and to provide the basic data for the sustainable management of Chinese fir plantation.[Method]Chinese fir seedlings were planted in sand with addition of different glyphosate levels (0, 2, 5, 10, 20, 50 mg·kg-1).Morphological and physiological features of Chinese fir were measured, and the nutrient content was determined.[Result]The results showed that under high concentrations of glyphosate, biomass of Chinese fir significantly decreased compared to the control (P<0.05), and fir leaves showed chlorosis.At glyphosate level of 50 mg·kg-1, seedlings all died.Physiological parameters of seedlings showed that increasing glyphosate levels led to increased relative conductivity, MDA content, SOD activity, and POD activity in leaves, and decreased proline and soluble protein content.Chlorophyll a, chlorophyll b and total chlorophyll content first rose then declined, while shikimic acid content showed no significant change.The macro and micronutrient accumulation in roots and leaves of Chinese fir seedlings were affected by glyphosate.With the increase in glyphosate, Cu, Ca and Mg levels increased in the roots, while there was no significant change in the accumulation of Mn, Fe and Zn.In addition, the accumulation of Cu in Chinese fir leaves increased and Mn increased first and then decreased, and Zn increased after decline.[Conclusion]Overall, we found that glyphosate had negative effect on Chinese fir growth, led to physiological disorder, disturbed plant nutrient absorption, and negatively influenced seeding growth and survival rate.Therefore our findings suggest that reducing herbicide dose for weed control would improve the yield of Chinese fir young plantations.关键词
杉木/草甘膦/生理响应/养分吸收Key words
Chinese fir/glyphosate/physiological character/nutrient absorption分类
农业科技引用本文复制引用
周垂帆,林静雯,李莹,刘爱琴..土壤残存草甘膦对杉木幼苗生理及养分吸收的影响[J].林业科学,2017,53(4):56-64,9.基金项目
国家自然科学基金项目(31400465) （31400465）
福建省自然科学基金项目(2015J05050) （2015J05050）
中国博士后科学基金项目(2015M570550). （2015M570550）
