南华大学学报（自然科学版）2017，Vol.31Issue(1)：85-90,6.
介孔硅材料组装DNA电化学生物膜电极的制备
Mesoporous Silica Nanoparticles to Assemble the Preparation ofDNA Electrochemical Biofilm Electrode
摘要
Abstract
A novel DNA electrochemical biofilm electrode was fabricated using Sulfo-SMCC as the heterobifunctional coupling agent which made the sulfhydryl-modified DNAzyme,covalently immobilized on the aminated mesoporous silica nanoparticles (MSN) loaded with the electrochemical indicator methylene blue (MB),and then mixed with chitosan (CTS) dropping applied to the carbon paste electrodes.The preparation process of the biofilm electrode was characterized by cyclic voltammetry (CV),electrochemical impedance spectroscopy (EIS) and so on.The results showed that the DNAzyme based on thiol-modified was successfully immobilized on the silica nanomaterials,and the prepared electrode material formed a stable film on the surface of the carbon paste electrode.关键词
二氧化硅纳米材料/DNAzymes/亚甲基蓝/电化学生物膜电极Key words
mesoporous silica nanoparticles/DNAzymes/methylene blue/DNA electrochemical biofilm electrode分类
化学化工引用本文复制引用
文艺,袁亚莉,李乐,廖琪,侯少岩..介孔硅材料组装DNA电化学生物膜电极的制备[J].南华大学学报（自然科学版）,2017,31(1):85-90,6.基金项目
国家自然科学基金项目(11405081) （11405081）
湖南省科技厅项目(2014FJ6097) （2014FJ6097）
