农业生物技术学报2017，Vol.25Issue(5)：689-699,11.DOI:10.3969/j.issn.1674-7968.2017.05.001
抗条锈病、耐盐小麦-十倍体长穗偃麦草5J(E)二体异附加系的分子细胞学鉴定
Molecular Cytogenetic Identification of Triticum aestivum-Thinopyrum ponticum 5J(E) Disomic Addition Line with Stripe Rust Resistance and Salt Tolerance
摘要
Abstract
Thinopyrum ponticum (2n=10x=70),which contains excellent resistance and stress tolerance genes to against biotic and abiotic stress,is an important germplasm for wheat (Triticum aestivum) improvement.The development of novel Tr.aestivum (common wheat)-Th.ponticum derivatives will provide valuable germplasm to wheat resistance breeding programs.CH 1115-B 15-1-5-1-1 (CH 1115-B 15),a novel disomic addition line from the cross of 7182 (Tr.aestivum)/Th.ponticum//87-1-9 (Tr.aestivum),was characterized by cytological identification,morphological analysis,salt tolerance analysis,genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) analysis,simple sequence repeat (SSR),expressed sequence tag (EST) and PCR based landmark unique gene (PLUG) markers analysis.Cytological observations showed that CH11 15-B 15 contained 44 chromosomes and formed 22 bivalents at meiotic metaphase Ⅰ and the addition line was stable in morphology and cytology.GISH and FISH analysis suggested that,of 44 line chromosomes,42 chromosomes were common with wheat chromosomes,and 2 chromosomes came from Th.ponticum chromosomes.In addition,the pair of alien chromosomes carried on similar Oligo-pTa535 signals.GISH analysis showed that the pair of alien chromosomes belonged to the genome J(E).The polymorphic analysis with 2 SSR markers,4 EST markers,and 4 PLUG markers showed that the pair of alien chromosome belonged to the fifth homeologous group.Salt tolerance analysis at the germination stage and evaluation of adult stripe rust resistance showed that line had high resistance to wheat stripe rust (IT=1) at the adult stage and excellent salt tolerance at the germination stage.Therefore,CH1115-B 15 is Tr.aestivum-Th.ponticum 5J (E) disomic addition line with excellent stripe rust resistance and salt tolerance,and it could be exploited as a promising germplasm in wheat breeding and chromosome engineering.关键词
小麦/长穗偃麦草/原位杂交(GISH)/抗条锈病/耐盐性/分子标记Key words
Triticum aestivum/Thinopyrum ponticum/Genomic in situ hybridization (GISH)/Stripe rust resistance/Salt tolerance/Molecular markers分类
农业科技引用本文复制引用
朱晨,王艳珍,陈春环,王长有,吉万全..抗条锈病、耐盐小麦-十倍体长穗偃麦草5J(E)二体异附加系的分子细胞学鉴定[J].农业生物技术学报,2017,25(5):689-699,11.基金项目
国家重点研发计划项目(No.2016YFD0102000)、陕西省科技统筹创新工程计划项目(No.2015KTZDNY01-01-02)和陕西省农业科技创新与攻关项目(No.2016NY-031) （No.2016YFD0102000）
