山东医药2017，Vol.57Issue(19)：17-20,4.DOI:10.3969/j.issn.1002-266X.2017.19.005
Matrigel三维细胞培养方法培养的原代乳腺癌细胞生长情况及对阿霉素敏感性观察
Growth of primary breast cancer cells cultured by Matrigel three-dimensional cell culture method and its sensitivity to adriamycin
摘要
Abstract
Objective To observe the growth of primary breast cancer cells cultured in Matrigel three-dimensional cell culture method and its sensitivity to adriamycin.Methods The primary breast cancer cells were cultured by two-dimensional monolayer culture and Matrigel three-dimensional cell culture methods.On the 1st, 3rd, 5th and 7th day of culture, Olympus IX70 inverted microscope was used to observe the morphology of primary breast cancer cells under the condition of two-dimensional monolayer culture and three-dimensional culture.CellTiter-Glo(R) Luminescent Cell Viability Assay was applied to observe the cell proliferation and its sensitivity to adriamycin.Primary breast cancer cell growth curve and drug sensitivity curve were drawn based on the observation results.Results The primary breast cancer cells with two-dimensional monolayer culture were in adherent growth, and the cancer cells gathered like a nest, with a variety of forms.The primary breast cancer cells with three-dimensional cell culture gathered into close multi-tumor cell microspheres.The proliferation of primary breast cancer cells cultured by two-dimensional monolayer culture on the 1st, 3rd, 5th and 7th day was 1.00±0.00, 1.39±0.29, 2.08±0.25 and 3.26±0.41.The proliferation of primary breast cancer cells cultured by Matrigel three-dimensional cell culture was 1.00±0.00, 1.25±0.08, 1.79±0.10 and 1.58±0.02.With the increase of adriamycin concentration, the inhibition rate of proliferation of primary breast cancer cells increased under two-dimensional monolayer culture and three-dimensional culture.But the proliferation inhibition rate of primary breast cancer cells with three-dimensional cell culture was lower than that with two-dimensional cell culture.Conclusion The primary breast cancer cells with Matrigel three-dimensional cell culture can gather into close spheres, the cell proliferation rate slows down, and the sensitivity of adriamycin decreases.Matrigel three-dimensional cell culture is better for culturing primary breast cancer cells.关键词
乳腺肿瘤/乳腺癌/Matrigel三维细胞培养方法/细胞形态/细胞增殖/药物敏感性Key words
breast neoplasms/breast carcinama/Matrigel three-dimensional cell culture method/cell morphology/cell proliferation/drug sensitivity分类
医药卫生引用本文复制引用
张震,齐晓敏,张薇,张培,曹旭晨,肖春花..Matrigel三维细胞培养方法培养的原代乳腺癌细胞生长情况及对阿霉素敏感性观察[J].山东医药,2017,57(19):17-20,4.基金项目
中国医学基金会肿瘤预防与科研项目(314.2222). （314.2222）
